Health Research Program – Opportunity Archive

Grace Nichols
HRP participant Grace Nichols '20 (CLAS) using software to measure response rates of mice with hopes of understanding tinnitus.

Opportunity Archive

The Health Research Program was first launched in Spring 2017. Cohorts of students were selected to start in the program in Spring 2017, Summer 2017, Academic Year 2017-18 (Fall 2017), Summer 2018, Summer 2019, Summer 2020, Summer 2021, Summer 2022, and Summer 2023. The opportunities that were offered in each of these past phases of the program are listed here for reference, but none of these are active opportunities. Information about the next phase of the Health Research Program - Summer 2024 - will be added to the main HRP webpage for students before the 2023-24 academic year begins.

Summer 2023 Research Opportunities

Summer 2023 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Corey Acker Center for Cell Analysis and Modeling This project is for the development of new and improved ways to study the electrical (action potential) signaling in cell culture models of disease. Custom fluorescence microscopy equipment is used along with patch clamp electrophysiology to control and monitor the voltage inside cells by recording voltage changes optically as well as electrically. Optical recordings will be applied to cell culture models including either human stem cell-derived heart cells or neurons. MATLAB is used for all aspects, from hardware control to data analysis. HRP SU23-1
Dr. Eran Agmon Center for Cell Analysis and Modeling, Department of Molecular Biology and Biophysics, Department of Biomedical Engineering For this summer project, the student will work on a computational model of bacterial interactions in the human gut microbiome. The student will write code to add new mechanistic details to the model of the microbiome, will simulate it, visualize the results, and evaluate the results by comparing with experimental data. The final model and results will be presented in a Python notebook and shared online for others to learn from. HRP SU23-2
Dr. Srdjan Antic Department of Neuroscience This is a basic science research project aimed at understanding cellular determinants of Alzheimer’s disease (AD). We use an animal model of AD -- mice carrying specific genes, which trigger development of amyloid plaques in the cerebral cortex. By the means of brain slice technology, electrophysiology (synaptic stimulation), and optical imaging (calcium and voltage imaging) we seek to determine if synaptic transmission in disease-free animals (Control) is any different from that occurring in the AD model animals developing amyloid plaques. The student will learn software for analysis of the optical imaging records (physiological), organization and presentation of measurements, some basic histology techniques for detecting plaques in saved brain sections, and if interested, the student may perform optical imaging of synaptically evoked depolarizations in brain slices working together with a postdoc. HRP SU23-3
Dr. Michael Blinov Center for Cell Analysis and Modeling, Department of Genetics and Genome Sciences Mathematical modeling of biological processes is important to gain an understanding of the underlying biological mechanisms and predict the dynamics and outcomes of experiments and medical interventions. Mathematical models describe interactions among components of biological systems. Models are implemented and simulated in the Virtual Cell (http://vcell.org) software using GUI. We will develop an infrastructure and a set of small models (ModelBricks, http://modelbricks.org) that serve as building blocks for larger models. HRP SU23-4
Dr. Melissa Caimano Departments of Medicine, Pediatrics, and Molecular Biology and Biophysics The project focuses on understanding how Lyme disease spirochetes alter their gene expression in response to tick and mammalian host signals. The summer research project will center on developing fluorescent (GFP) transcriptional reporters to localize DNA binding sites for regulatory proteins related to virulence. These constructs then would be transferred in spirochetes. If time allows, gene expression will be measured by microscopy and flow cytometry. HRP SU23-5
Dr. Leslie Caromile Center for Vascular Biology, Department of Cell Biology The overall objective of this study is to develop a well-defined 3D bioprinted in vitro/in vivo model that mimics the molecular, cellular, and metabolic interplay occurring in the bone-tumor microenvironment of metastatic prostate cancer and confirm that it is responsive to targeted and non-targeted diagnostic and therapeutic modalities. HRP SU23-6
Dr. Manuel Castro-Alamancos Department of Neuroscience The project is for students in the fields of data science, engineering, computer science, applied math, statistics, psychology, or neuroscience that have programming skills in Python, R, and/or Labview and who are interested in helping develop tools for automated analysis of complex data pipelines originating from neuroscientific experiments addressing the neural basis of behavior in mice. The student would be involved in developing the software tools used to control the experiments (actuators and computer vision) and analyze the data from these experiments (data models). Opportunities to also participate in the research by conducting experiments are also available. HRP SU23-7
Dr. Steven Chou Department of Molecular Biology and Biophysics Using the red blood cell cytoskeleton to probe the structural basis of actin polymerization by formins. Aim 1. Isolation of the short actin filaments in human red blood cells. Aim 2. Architecture of short actin filaments alone. Aim 3. Association of FH2 domains to the short actin filaments. Aim 4. Structural basis of actin nucleation and polymerization by different FH2 domains. Aim 5. Inhibition of pathological Formin-mediated actin polymerization. HRP SU23-8
Dr. Jessica Costa Center for Molecular Oncology, Center for Regenerative Medicine and Skeletal Development Our research focuses on modeling of parathyroid tumorigenesis and the related Hyperparathyroidism-Jaw Tumor syndrome. The student will assist in phenotypic characterization of genetically modified mice including: assisting in mouse colony management, blood collection, tissue harvest, rotary sectioning of fixed tissue, immunohistochemistry and measurement of serum calcium and PTH. Student will also be involved in primary culture of harvested tissues. HRP SU23-9
Dr. Caroline Dealy Departments of Craniofacial Sciences, Biomedical Engineering, Orthopedic Surgery, and Cell Biology Diagnostic tests that accurately reflect cellular physiology are critical for predicting patient response to cancer drugs. Our group has identified a human blood protein whose levels in circulation correlate with aging and cancer. Surprisingly, this circulating protein is produced via alternate splicing of a gene that previously was only known to produce a membrane-bound protein. The membrane-bound protein has multiple roles in development, health, and disease and is the target of several major cancer drugs; however, the efficacy of these drugs is variable and does not correlate with tumor expression of the membrane-bound protein. We propose this is because available diagnostic tests fail to account for the circulating isoform, which we believe regulates the function of the membrane-bound protein. Indeed, we have found that the relative proportions of the two alternatively-spliced transcripts vary across a wide range of normal and developing tissues in mice, suggesting that production of the different protein isoforms is tightly regulated and may relate to specific functions carried out by different tissues. The goal of this summer project is to correlate the relative proportions of the alternately-spliced transcripts to the relative amounts of the two protein isoforms they encode. Approaches will include quantitative PCR, Western Blot, and ELISA. The information obtained will be first-of-its-kind and is critical for foundational understanding of the physiological role of the circulating protein and its potential as a future cancer diagnostic. HRP SU23-10
Dr. Anne Delany Department of Medicine, Center for Molecular Oncology The goal of this summer project is to identify novel mRNA targets for microRNAs (miRNAs, small noncoding RNAs that negatively regulate gene expression). Students will learn key aspects of miRNA and bone cell biology. Regarding technical skills, students will learn to: clone specific DNA fragments from mouse genomic DNA into a plasmid vector, transform bacteria, isolate plasmid DNA, perform diagnostic restriction endonuclease analysis, culture and transfect mouse cell lines, perform luciferase assays, and critically analyze data. Students may learn to isolate RNA and perform qRT-PCR. HRP SU23-11
Dr. Alix Deymier Department of Biomedical Engineering Your teeth are made up of 2 main parts: enamel and dentin. These 2 very different materials are connected to each other via a very unique interface called the Dentin-Enamel Junction (DEJ). In theory, the DEJ should be a very weak interface, but instead it is extremely fracture resistant in healthy individuals. One the other hand, people with certain genetic diseases like osteogenesis imperfecta (OI), brittle bone disease, have very weak DEJs. Our goal is to compare the structure and composition of DEJs from teeth that are healthy and those that have OI to understand what gives this interface its unique properties. HRP SU23-12
Dr. Zhichao Fan Department of Immunology This project will work on small molecular drug screening to identify potential drugs to inhibit neutrophil recruitment during inflammatory diseases. We have accomplished a drug screening and found several hits for further exploration. The summer student will work with a graduate student on this project to confirm the effects of hits on inhibiting neutrophil integrin activation and adhesion. In vivo experiments on mice might be performed to demonstrate physiological relevance. HRP SU23-13
Dr. Mallika Ghosh Department of Cell Biology, Center for Vascular Biology Cell-cell fusion is a highly specialized process mediated by fusion regulatory proteins involved in a variety of diverse cellular function. CD13, a transmembrane protein that we study in my lab, controls many events including adhesion, migration, membrane organization, and endocytosis that are all critical in cell-cell fusion. We hypothesize that CD13 is a negative regulator of cell-cell fusion during osteoclastogenesis and macrophage giant cell formation, and thus is a novel target for therapeutic intervention in pathological conditions mediated by defects in cell-cell fusion such as osteoporosis and failure of medical implants such as hernia mesh or glucose sensor. HRP SU23-14
Dr. Carolyn Greene Department of Psychiatry I am seeking an undergraduate student researcher to assist with the Cultural Understanding of Emotions Study (CUES) a research study investigating emotion socialization processes in Latinx families. The student researcher will assist with the cultural and linguistic adaptation and piloting of a measure of parental emotion socialization behaviors, and will also assist with implementation of a national on-line survey, as well as with other study tasks. The student researcher will have the opportunity to develop a poster or paper on a research question of their choosing, related to children’s emotion regulation, parents’ socialization behaviors, and children’s functioning. Data for the project can be drawn from (1) qualitative CUES study data from focus groups of Latinx parents discussing emotion processes in their families, or (2) data from a previous study on parent and child emotion regulation and emotion socialization. The student researcher will be expected to attend research team meetings. HRP SU23-15
Dr. Xiaoyan Guo Department of Genetics and Genome Sciences The student will have the opportunity to study the mitochondrial stress response signaling pathway in mammalian cells. Previous work from the lab has identified a novel pathway with OMA1-DELE1-HRI to relay mitochondrial dysfunction to the cytosol/nucleus. DELE1 is a messenger protein from dysfunctional mitochondria, which we still know little about. This summer project will focus on how DELE1 is imported to mitochondria using cell biology, molecular biology, and biochemistry approaches. HRP SU23-16
Dr. Christopher Heinen Department of Medicine, Center for Molecular Oncology Our lab uses CRISPR gene editing to recreate patient variants in the DNA mismatch repair genes in a human stem cell model system. The goal is to study the functional effects of these variants. Summer students will initially learn some molecular biology techniques to assist in validating the creation of these variant cell lines. As the project goes forward, there is the opportunity to learn some human cell culture techniques and perform some CRISPR editing. HRP SU23-17
Dr. Sivapriya Kailasan Vanaja Department of Immunology The primary focus of our research is to determine the mechanisms by which bacterial pathogens modulate host innate immune responses. Since innate immune responses play a crucial role in the clearance of infectious agents, it is natural that pathogens have developed strategies to inhibit the same. Our research aims to identify bacterial mechanisms that inhibit innate immune activation. The summer research opportunity will focus on identifying virulence factors of two enteric pathogens, Salmonella Typhimurium and Enterohemorrhagic E. coli, that can suppress a major innate immune pathway, the inflammasomes. The research strategy will involve working with bacterial mutants that lack specific virulence factors and determining their mode of action in innate immune cells such as macrophages. HRP SU23-18
Dr. Adam Kim Department of Medicine Our lab is interested in understanding how the immune system contributes to liver disease by utilizing patient samples, next-generation sequencing technologies, and bioinformatic analyses. In this project, we will use peripheral blood mononuclear cells (PBMCs) isolated from patients with alcohol-associated hepatitis, challenge them with bacterial LPS, then perform single-cell RNA-seq to understand gene expression responses in different immune cell types. By using PBMCs isolated from males and females, we investigate sexual dimorphism in immune cell function and how that might contribute to disease. HRP SU23-19
Dr. Kshitiz Department of Biomedical Engineering We are exploring many parallels between cancer malignancies and pregnancy bringing many aspects of biology and medicine together. There are multiple interesting angles from which we approach these questions, involving integration across disciplines, so there is something for you whatever your interest. These include biophysics, animal work, tissue engineering, microscopy, computation, machine learning, bioinformatics, and preclinical projects. Specifically, we look at how cancer cells interact with stromal (non cancerous, non immune) cells, and in a similar vein how fetal cells interact with maternal cells. There are surprising similarities between the two very different phenomena and we will explore these in our various mini projects. HRP SU23-20
Dr. Sangamesh Kumbar Department of Orthopedic Surgery The project involves formulation and evaluation of sprayable/injectable bioengineered polymeric scaffolds for wound healing and drug-delivery application. Briefly, the student researcher along with the mentors will optimize the formula for the polymeric scaffold. Furthermore, the student researcher will use the formula to prepare the scaffolds which will then be evaluated to determine a variety of physical, chemical, and biological properties of the scaffold. The student researcher selected in this project will have the opportunity to learn various techniques of formulation of bioengineered scaffolds and in-vitro animal cell culture and understand all of the aseptic methods utilized in animal cell culture. HRP SU23-21
Dr. James Li Department of Genetics and Genome Sciences We study how the brain develops and how abnormal development contributes to brain disorders. We are using a number of approaches, including single-cell genomics and epigenomics, molecular biology, and experiments in stem cells and animal models (mouse and chick), to study the generation of various neurons and the assembly of neural circuitry, particularly in the cerebellum. Our multidisciplinary study will provide students with ample opportunities to explore different research approaches and identify a project that aligns with the individual’s skill, interest, and career goal. HRP SU23-22
Dr. Changchun Liu Department of Biomedical Engineering Nucleic acid-based molecular detection provides a highly sensitive and specific approach for rapid diagnostics of infectious diseases (e.g., COVID-19). In this summer project, we will design and fabricate microfluidic chips to detect nucleic acid molecules (e.g., DNA, RNA). We will use 3D printing technology to fabricate the chips. Please find more detail on our current research through the website: https://smds.engr.uconn.edu/. HRP SU23-23
Dr. Leslie Loew Center for Cell Analysis and Modeling, Department of Cell Biology The lab is using computational methods to understand cell function. The project will involve learning how to use a cell modeling software tool that was developed in my lab called SpringSaLaD (https://vcell.org/ssalad). You will use it to understand the molecular and cellular principles controlling the assembly of molecular machines composed of many individual molecules containing multiple binding sites. If you are interested in computer programming, there will also be the opportunity to work on improving SpringSaLaD. HRP SU23-24
Dr. Kevin Manning Department of Psychiatry, Center on Aging Older adults lost more than $1.7 billion to fraud last year. The current project aims to understand the cognitive, motivational, and emotional symptoms that contribute to financial decision making and susceptibility to scams. The student will learn to administer cognitive evaluations, conduct telephone screens asking about depression symptoms and history, and administer assessments of financial literacy and decision making to adults over the age of 60. Students will also learn about and contribute to data entry using databases like Redcap, Access, and SPSS. HRP SU23-25
Dr. Bruce Mayer Department of Genetics and Genome Sciences, Center for Cell Analysis and Modeling We work on new ways to visualize cell signaling pathways that are active in cells, particularly in cancer cells. We have developed a method based on the binding of fluorescent protein domains that can be used to quantify the tyrosine kinase signaling activity in single cells, based on fluorescence microscopy and flow cytometry. While the basic platform of the assays is well established, there is a great deal of work needed to optimize the method and validate it on different types of cell samples. This will provide the student with experience in assay and technology development, basic biochemistry skills, and cancer research. My group works closely with two other faculty, Dr. Kazuya Machida and Dr. Ji Yu, on developing these new assays. HRP SU23-26
Dr. Pedro Miura Department of Genetics and Genome Sciences This summer research opportunity would involve a mix of mammalian cell culture work, molecular biology, and next-generation sequencing dataset analysis (short-read, long-read and single cell RNA-Seq). The Dnmt3a gene generates two alternative length 3’UTRs in mouse and human neurons. The student will investigate the biological role of the long 3’UTR Dnmt3a isoform by analyzing CRISPR-deletion ES cell lines differentiated into glutamatergic neurons. HRP SU23-27
Dr. Megan O'Grady Department of Public Health Sciences The State of Connecticut has recently legalized recreational cannabis for adults. This change in policy may have impacts on public health, making it imperative that data be monitored and analyzed to study the impacts on youth and adult health across the State. This summer research opportunity will support the Center for Prevention Evaluation and Statistics at UConn Health in a research project related to cannabis legalization, specifically by contributing to a systematic review and policy analysis of data monitoring for adult cannabis legalization and preparation of a manuscript describing the findings. HRP SU23-28
Dr. Stefan Pinter Department of Genetics and Genome Sciences The Pinter lab studies genetic syndromes that change the expression levels of many genes residing on a single chromosome, for example Down syndrome (DS, trisomy 21) and Turner syndrome (TS, monosomy X). We have established human stem cell (iPSC) lines of these aneuploidies, along with isogenic euploid control lines, to model cellular phenotypes associated with these conditions. To correct the dosage of genes on chromosome X or 21, we use epigenetic (XIST RNA) and CRISPR tools to study their developmental impact, and map cellular phenotypes back to specific mis-expressed genes, for example, in our current preprint: https://www.biorxiv.org/content/10.1101/2022.05.11.491519v1. For the summer of 2023 we have both in-person research opportunities in cellular characterization of iPSC-derived cell types, as well as hybrid research opportunities in bioinformatics analysis of transcriptomic or high-content imaging data. Note: The Health Research Program has certain in-person participation requirements, including a presentation at Summer Research Day, that a student working in a hybrid arrangement would be expected to satisfy. HRP SU23-29
Dr. Carla Rash Department of Medicine My lab does behavioral research related to treatment and behavior change, mostly around substance use disorder and/or interventions for severely disadvantaged groups. I have two ongoing projects: 1) an NIH-funded clinical trial that aims to use contingency management to help persons living with HIV/AIDS obtain employment; and 2) a SAMHSA-funded project that expands an existing medication-assisted treatment program for persons with opioid use disorders. These two projects are both located in Hartford and would provide opportunities to learn about working with clients, clinical trials, and conducting participant interviews. In addition, for those who are interested, I have finished studies that would be available for data analyses and possible submission of posters to local and/or national conferences. HRP SU23-30
Dr. Blanka Rogina Department of Genetics and Genome Sciences The Indy (I’m Not Dead Yet) gene encodes a plasma membrane citrate transporter. We have shown that reduced expression of the Indy gene extends longevity in fruit flies by altering energy metabolism and preserving metabolic homeostasis. INDY regulates citrate levels, which occupies a central role in the regulation of glycolysis, lipid synthesis, and energy flux in the mitochondrion. Alzheimer’s disease (AD) is a progressive, age-associated neurodegenerative disease characterized by cognitive impairment, and other mental functions. AD is associated with altered metabolism and impaired mitochondrial function. We are testing our working hypothesis that preservation of metabolic and mitochondrial function associated with Indy reduction will ameliorate metabolic imbalance associated with AD. We are using a fly model of human AD, which contains an amyloid precursor protein mutation associated with early onset AD in a Swedish pedigree (APPswe), and are investigating the effects of Indy reduction on metabolic, transcriptomic, and epigenetic changes associated with AD. HRP SU23-31
Dr. Archana Sanjay Department of Orthopedic Surgery The ongoing work in my laboratory focuses on characterizing skeletal stem/progenitor cells. We have developed several transgenic mouse lines. The summer research project will be to assist in characterizing these mouse lines using a variety of techniques including: frozen sectioning of bones, staining and imaging sections, gene expression by qRTPCR, and cell cultures. HRP SU23-32
Dr. Tannin Schmidt Department of Biomedical Engineering Lubricin is a multifunctional protein, with both lubricating and anti-inflammatory properties, that is present throughout the human body. While initially discovered and studied in synovial fluid as a lubricant of joints, we recently discovered it in tears, the ocular surface, and in the posterior of the eye. Recombinant human lubricin has been shown to be clinically effective in treating dry eye disease, improving signs and symptoms of dry eye disease in humans. This project will examine the biological (anti-inflammatory) properties of recombinant human lubricin in the context of disease(s) related to the eye. HRP SU23-33
Dr. Henry Smilowitz Department of Cell Biology Our lab, in collaboration with a small biotech company, has pioneered the use of non-toxic heavy atom nanoparticles (NPs) to enhance radiation therapy (RT) of tumors. Projects will involve proof of concept studies of the efficacy and microlocalization of both the INPs and a novel NP with exciting new properties for the treatment of tumors with an emphasis on brain tumors. Another project will study a novel NP for the delivery of inhibitory RNAs and/or microRNAS in combination with drugs to tumors--with an emphasis on brain tumors. We also have a atherosclerosis project using heavy atom nanoparticles to study atherosclerotic plaque progression and regression in live mice over time. Please see our cell biology blurb for our publications and research interests. HRP SU23-34
Dr. Timothy Spellman Department of Neuroscience The prefrontal cortex is an area of the brain that is critically involved in processing attention, short-term memory, and rule learning. This project seeks to clarify the involvement of specific neurotransmitters, including acetylcholine and dopamine, in allowing the cells of the prefrontal cortex focus attention on external cues, and to shift that focus when necessary. These are cognitive processes that are disrupted in a wide range of psychiatric diseases, which we are seeking to better understand. Students would be involved in carrying out behavioral neuroscience experiments, which will entail testing mice on tasks involving attention, and also using several different cutting-edge techniques for monitoring and manipulating the activity of specific cell types in the prefrontal cortex of the mice undergoing these tests. HRP SU23-35
Dr. Ali Tamayol Department of Biomedical Engineering We are developing a robotic bioprinter that can directly bioprint scaffolds within a patient's body. As part of this effort, the candidate will work with our team to integrate the robot with a moving system that allows it to travel across the patient bed to cover different patient body parts. The system will be programmed and the quality of bioprinting will be tested in vitro. HRP SU23-36
Dr. Ephraim Trakhtenberg Department of Neuroscience We study how the brain develops and utilize gained knowledge to reverse-engineer regeneration of the brain tissue damaged by an injury or stroke. We employ a multidisciplinary approach spanning cutting edge genetics, epigenetics, bioinformatics, molecular biology, and gene therapy, which will provide a student with an opportunity to explore different approaches and select a project that aligns best with the individual’s career goals and interests. HRP SU23-37
Dr. Melanie Tran Department of Nephrology Chronic kidney disease is associated with accelerated and premature ageing of the kidney, which is characterized by cellular senescence. Moreover, accelerated tubular cell senescence promotes the pathogenesis of renal fibrosis. This research project aims to investigate signaling pathways that regulate cell senescence and renal fibrosis development. The student will gain experience in cell culture, immunofluorescence, immunohistochemistry, qPCR, Western blot, and mouse models of chronic kidney disease. HRP SU23-38
Dr. Oscar Vargas-Rodriguez Department of Molecular Biology and Biophysics The summer research project aims to decipher the functional role of a family of proteins known as aminoacyl-tRNA synthetases in bacterial pathogenicity. These proteins regulate protein biosynthesis and may enable pathogenic microbes to survive, adapt, and thrive in different environments, particularly within their host during colonization and infection. The student will perform standard molecular biology and biochemistry assays, including molecular cloning, protein, nucleic acid manipulation, enzymatic assays, and cell-based experiments. HRP SU23-39
Dr. Paola Vera-Licona Center for Cell Analysis and Modeling, Center for Quantitative Medicine This is a computational-based project where the student will identify combinations of perturbation to induce cell reprogramming in pancreatic beta cells. The student will have the opportunity to learn how to (1) use multi-omics profiles, including RNA-sequencing data, to reconstruct a signaling network of the biological system of interest, (2) apply our in-house and other computational tools for cell-reprogramming analysis, and (3) discuss and interpret the results and future steps, together with our collaborators. HRP SU23-40
Dr. Rajkumar Verma Department of Neuroscience Encephalomyosynangiosis (EMS) is a neurosurgical procedure with low morbidity that is applied to promote collateral vascular formation in patients with moyamoya disease, a condition with progressive narrowing of cranial arteries and consequent low blood flow that increases risk for ischemic stroke. Therefore, we hypothesized that EMS, which provides a local and robust tissue as a source of vascular endothelium and angiogenic growth factors, will supply growth factors for angiogenesis that could promote neuronal survival following ischemic stroke. As a proof of concept, we have established EMS surgery for the first time in mice after ischemic stroke. The student will be helpful in investigating EMS for treating ischemic stroke for the following overall goals: to determine if EMS promotes angiogenesis after a ischemic stroke, and to determine if EMS promotes long-term functional recovery after ischemic stroke. HRP SU23-41
Dr. Kepeng Wang Department of Immunology My lab is developing novel oncolytic viral therapies for advanced cancer. The project aims to generate a new viral platform that, when delivered systemically, homes to tumor in a specific manner. On top of the new platform, we are testing different immune modulating payloads to activate cancer-killing immune cells and bypass immune suppression. The student will generate new oncolytic viral agents, and test their safety and efficacy on mouse models of cancers. HRP SU23-42
Dr. Zhao-Wen Wang Department of Neuroscience We recently discovered that melatonin promotes sleep in the nematode C. elegans by activating the SLO-1 potassium channel through a specific melatonin receptor, PCDR-1 (Niu et al., PNAS 117: 25128-25137, 2020). This project is to identify the neurons where PCDR-1 and SLO-1 act to regulate sleep by analyzing activities of neurons in the C. elegans sleep neural circuit using a genetically encoded calcium indicator, and by correlating neuronal activities with worm sleep behavior. HRP SU23-43
Dr. Ping Yan Center for Cell Analysis and Modeling There is a research opportunity on developing new voltage sensitive dyes, which are used for imaging membrane potentials. The student will have broad hands-on experience on the synthesis of organic compounds, and test the newly developed voltage sensitive dyes using spectroscopic and microscopic methods, on model cell membrane, and even on real cells (in collaboration with Dr. Loew). HRP SU23-44
Dr. Ji Yu Center for Cell Analysis and Modeling, Department of Genetics and Genome Sciences Our lab specializes in single-cell analysis of the cancer phosphoproteome. The specific student project involves acquiring profiling data on a subset of PBMC samples collected from chronic lymphocytic leukemia (CLL) patients. The student will: (1) Initiate the sample preparation pipeline and perform quality control evaluation; (2) Operate the automatic phosphoproteome profiling instrument to acquire data from the samples; (3) Perform quantitative evaluation of data quality. HRP SU23-45
Dr. Kristyn Zajac Department of Medicine, Calhoun Cardiology Center The Collaborative Hub for Emerging Adult Recovery Research (CHEARR) at the UConn School of Medicine is recruiting students interested in learning skills to conduct research on recovery support services for emerging adults (ages 18-25) with substance use disorders, with a particular focus on opioid use disorder. CHEARR activities include establishing and collaborating with community boards consisting of emerging adults in recovery and recovery coaches providing services in community settings; developing a range of technical tools to advance the research on recovery support services and communicate these advancements to other researchers, professionals, and the public (e.g., tipsheets, webinars, social media content); developing and validating a measure of recovery capital specifically for emerging adults; and training the next generation of researchers in the field of recovery supports. The student researcher would have the opportunity to participate in all of the above activities as well as two ongoing randomized clinical trials led by Drs. Zajac and Kelly that are relevant to this research area: 1) a clinical trial evaluating the use of recovery coaches to reduce treatment dropout among emerging adults in community-based substance use treatment, and 2) the development and evaluation of an mHealth tool for use by emerging adults who present to the emergency department following an alcohol- or suicidality-related medical crisis. HRP SU23-46
Dr. Misti Zamora Department of Public Health Sciences The primary project objective is to investigate how an individual’s choices influence personal exposures to traffic-related air pollutants (TRAPs) and the corresponding acute health effects. During the two 48-hour sampling periods, real-time air pollution data will be measured using an instrument that my team created. These measurements will be complemented with assessments of blood pressure, lung function, and pulmonary inflammation of the lower airways. We will work together to understand how people's exposures to air pollution impacts their health by collecting and analyzing this information for 65 participants (ongoing throughout the next year). HRP SU23-47
Dr. Yanjiao Zhou Department of Medicine My lab is a computational biology lab. We are interested in understanding the role of the gut microbiome and microbial metabolites in aging. The techniques we use include mouse models, anaerobic culture, molecular biology, and multi-OMICS technology. The student will have the opportunity to learn wet bench techniques and bioinformatics, depending on their interests. HRP SU23-48

Summer 2022 Research Opportunities

Summer 2022 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Corey Acker Center for Cell Analysis and Modeling The student will support testing of new voltage-sensitive dyes using cell culture assays. Custom fluorescence microscopy equipment is used along with patch clamp electrophysiology to control and monitor the voltage inside cells, including human stem cell derived heart cells, by recording voltage changes optically as well as electrically. MATLAB is used for all aspects, from hardware control to data analysis. HRP SU22-1
Dr. Thomas Agresta Department of Family Medicine and Center for Quantitative Medicine Medication Reconciliation is a complex process requiring access to information from a variety of Electronic Health Records, a patients or caregivers knowledge of what is being given in a home or what is administered in a hospital or skilled nursing facility. We seek students with skills and knowledge in user-centered design, pharmacy, nursing and Computer Science to assist in further development of prototypes to collect, analyze and share medication data to optimize care outcomes. This project will involve qualitative and quantitative research on design options and continued modification and development of user-facing mobile health tools. HRP SU22-2
Dr. Srdjan D Antic Department of Neuroscience This is a basic science research project aimed at understanding cellular determinants of Alzheimer’s disease (AD). We use an animal model of AD -- mice carrying specific genes, which trigger development of amyloid plaques in the cerebral cortex. By the means of brain slice technology, electrophysiology (synaptic stimulations), and optical imaging (calcium and voltage imaging) we seek to determine if synaptic transmission in disease-free animals (Control) is any different from that occurring in the AD model animals developing amyloid plaques. The student will learn software for analysis of the optical imaging records (physiological), organization and presentation of measurements, some basic histological techniques for detecting plaques in saved brain sections, and if interested, the student may perform optical imaging of synaptically-evoked depolarizations in brain slices working together with a postdoc. HRP SU22-3
Dr. Margaret Briggs-Gowan Department of Psychiatry This coming summer, our lab will be conducting multiple studies to learn about how stress and violence affect infants and young children. The first study is an NIMH-funded study investigating the effects of domestic violence on young children, ages 4-6 years. The summer intern will have the opportunity to assist with study activities which include developmental testing, computerized activities that assess children's processing of facial and auditory stimuli, puppet interviews, and in-depth interviews with mothers about family life and the child's development, posttraumatic stress, and well-being. Psychophysiological data are acquired during visits including event-related brain potentials. The second is investigating how stress associated with the pandemic is affecting infants born during the pandemic. This study will involve multiple methods, including surveys, interviews, videotaped assessments, and DNA samples. The summer intern will be an integral member of our study team and will have ample opportunity to get hands on research experience in these studies. HRP SU22-4
Dr. Melissa Caimano Department of Medicine The project would be related to understand how Lyme Disease spirochete (Borrelia burgdorferi) modulates gene transcription during its tick-mouse enzootic life cycle. Specifically, the project would involve identification of promoter elements that promote differential expression of virulence determinants required for survival in ticks or mice. The work will involve use of either fluorescent reporter constructs or DNA binding assays. HRP SU22-5
Dr. Manuel Castro-Alamancos Department of Neuroscience The project is for students in the fields of data science, engineering, computer science, applied math, statistics, psychology, or neuroscience that have programming skills in Python, R, and/or Labview who are interested in helping develop tools for automated analysis of complex data pipelines originating from neuroscientific experiments addressing the neural basis of behavior in mice. The student would be involved in developing the software tools used to control the experiments (actuators and computer vision) and analyze the data from these experiments (data models). HRP SU22-6
Dr. Jennifer Cavallari Department of Public Health Sciences We are seeking a student to join the Total Teacher Health research team studying the factors that influence educator stress and implementing workplace solutions to improve teacher mental well-being. You will learn many aspects of Public Health research including qualitative and quantitative data management and analysis as well as creation and testing of program materials. You will also gain knowledge in Public Health, community-based-participatory research, and occupational health and skills in survey research methods, and data management and analysis. HRP SU22-7
Dr. Caroline Dealy Department of Craniofacial Sciences/Cell Biology/Orthopedic Surgery/Biomedical Engineering The long term goal of this project is to develop a treatment for Osteoarthritis, a painful and debilitating disease caused by loss of articular cartilage of the joints (eg, knee or jaw). We have found that BMP and EGFR signals act antagonistically to control cartilage growth. We want to better understand this interaction so we can one day stimulate re-growth of articular cartilage lost in osteoarthritis. We found that mice that lack BMP develop osteoarthritis. We are testing the hypothesis that EGFR signals are upregulated in these mice, and that this is involved in cartilage loss. For this purpose this summer project will quantify levels of EGFR signaling and other cellular responses in the knee and/or jaw cartilage of these mice, using immunohistochemistry, image analysis and statistical analysis. Most of the tissue is already available; work with living animals will not be required. After the project is complete, the student will learn how to prepare and present a research poster which will be given at Frontiers and possibly at an additional nearby conference. HRP SU22-8
Dr. Alix Deymier Department of Biomedical engineering This study will focus on elucidating the mechanisms by which acid infiltrates bone and how it affects the bone porosity. We have shown that acid exposure seems to preferentially affect the bone exterior versus the bone interior. Therefore we will be using high resolution microCT, fluorescent dye kinetics and other imaging techniques to try and understand how acid gets into bone and what those consequences are. HRP SU22-9
Dr. Breno Diniz Department of Psychiatry and Center on Aging This research project aims to investigate the role of immunosenescence in aging and depressive symptoms in older adults. More specifically, we are investigating if older adults with depression have changes in inflammatory markers and immune cells phenotypes that are commonly observed during the pathological or accelerated biological aging processes. We also investigate if these immunological abnormalities are related to health outcomes in older adults with depression. In this project, we evaluate the inflammatory markers in the plasma using state-of-art multiplexing analyses. Immune cell phenotyping will be done using flow cytometry and immunohistochemistry. HRP SU22-10
Dr. Jennifer Garza Department of Medicine I am seeking a summer intern to participate in my research on promoting musculoskeletal health of older adults. You would be part of a longitudinal research study in which we have collected multiple measurements of strength, flexibility, and endurance over a 15-year period. Your responsibilities would include analysis of trends in the musculoskeletal health data over time. There may be some opportunities to assist with data collection as well. HRP SU22-11
Dr. Mallika Ghosh  Center for Vascular Biology Cell-cell fusion is a highly specialized process mediated by fusion regulatory proteins involved in a variety of diverse cellular function. CD13, a transmembrane protein that we study in my lab, controls many events including adhesion, migration, membrane organization and endocytosis that are all critical in cell-cell fusion. We hypothesize that CD13 is a negative regulator of cell-cell fusion during osteoclastogenesis and macrophage giant cell formation, and thus is a novel target for therapeutic intervention in pathological conditions mediated by defects in cell-cell fusion such as osteoporosis and implant failure. HRP SU22-12
Dr. Damion Grasso  Department of Psychiatry The summer research experience in the Family Adversity & Resilience Research (FARR) program involves two large studies funded by the National Institute of Health. The first is the Adaptation & Resilience in Childhood Study (ARCS), which seeks to understand what factors help to determine the link between early exposure to family violence and developing psychopathology in children ages 4-6. Activities related to this study include (1) facilitating in-person laboratory visits with families (e.g., conducting electroencephalograms, managing data acquisition, recording parent-child interactions), (2) assisting with participant correspondence, (3) laboratory visit preparation, and (4) recruitment efforts in the field. The second study is the Parenting Infants in the Pandemic Study (PIPS), which seeks to understand the impact of the pandemic on families who gave birth during the pandemic. This study involves an online survey and a remote interview where we will be conducting an interview, facilitating a parent-infant observation, and collecting DNA via a home kit. Students on this study will likely assist with participant correspondence, data management, remote visit preparation, and recruitment. Link to these research studies from our FARR page: h.uconn.edu/farr HRP SU22-13
Dr. Carolyn Greene  Department of Psychiatry I am seeking an undergraduate student intern to assist with the Parent and Child Emotions Study (PACES) a research study investigating the role of emotion regulation and socialization of emotion in the intergenerational transmission of mental health problems among parents and children who have experienced trauma. The intern will assist with parent and child visits to our laboratory, where families will be completing questionnaires and engaging in dyadic tasks during which we will collect physiological data, and will also assist with other study tasks. The intern will have the opportunity to utilize study data to develop a poster or paper on a research question of their choosing, related to children’s emotion regulation, parents’ socialization behaviors, and children’s functioning. The intern will be expected to attend research team meetings. HRP SU22-14
Dr. Christopher Heinen  Department of Medicine Our laboratory studies the hereditary disease Lynch syndrome in which patients have a greatly increased risk of developing colorectal and other cancers. Lynch syndrome is caused by germline mutations in one of the DNA mismatch repair genes. The current project aims to examine the effects of patient-derived missense variants on mismatch repair function in a human stem cell model. We are using CRISPR gene editing to create patient variants in the MSH6 mismatch repair gene found in patients suspected of having Lynch syndrome. HRP SU22-15
Dr. Mayu Inaba-Oguro  Department of Cell Biology The project aims to define factors localize to adherens junction (AJ) in an age-dependent manner. Summer student project is to generate transgenic flies to express proteins localize to adherens junctions (E-Cadherin and β-catenin) fused to a peroxidase (ascorbic acid peroxidase; APEX). Using these transgenic flies, we can perform Proximity-Labeling Mass Spectrometry (MS) to identify proteins localize to AJs using Drosophila ovaries isolated from young and old flies. HRP SU22-16
Dr. Liisa Kuhn  Department of Biomedical Engineering This project is about designing and making 3D printed breast prostheses for women that have had cancer and then a mastectomy. These prostheses are non-implantable. The student will learn how to design the objects from 3D images of patients using free software and then will learn how to make the prostheses by 3D printing an elastomeric material using a large 3D printer. The prostheses are given to actual volunteers that are wearing the forms and providing feedback so that Dr. Kuhn and her team can optimize the longevity, comfort and fit. HRP SU22-17
Dr. Changchun Liu  Department of Biomedical Engineering Rapid detection of pathogens plays a critical role in diagnostics of infectious diseases (e.g., COVID-19) (Ding, X. et al., Nature Communications, 2020, 11, 4711). In this summer project, we will develop microfluidics technology for DNA detection. The microfluidic device will be fabricated by using 3D printing technology. Please find more detail on our current research through the website: https://smds.engr.uconn.edu/. HRP SU22-18
Dr. Leslie Loew  Center for Cell Analysis and Modeling The lab is using computational methods to understand cell function. The project will involve learning how to use a cell modeling software tool that was developed in my lab called SpringSaLaD (https://vcell.org/ssalad). You will use it to understand the molecular and cellular principles controlling the assembly of molecular machines composed of many individual molecules containing multiple binding sites. If you are interested in computer programming, there will also be the opportunity to work on improving SpringSaLaD. HRP SU22-19
Dr. Pedro Mendes Department of Cell Biology Iron Regulatory Proteins 1 and 2 (IRP1 and IRP2) are at the center of cellular iron regulation and though there is a wealth of experimental data surrounding these regulators and their targets, no quantitative models exist. The project will consist of developing a computational model of IRP regulation using published experimental data and the COPASI modeling software. The objective is to explore the quantitative nature of IRP regulation on their targets, particularly ferritin, the iron storage protein. The model will include the dynamics of 1) ferritin transcription, translation, and degradation, 2) the synthesis and degradation of IRP1/2, 3) IRP1/2 post-translational repression of ferritin synthesis, and 4) IRP1/2 inhibition by cytosolic iron. This project will allow the student to learn how to develop and use mathematical models of biological systems. HRP SU22-20
Dr. Mina Mina  Division of Pediatric Dentistry The goal of all research project in my laboratory is improving the natural healing process of dentin ad pulp in the tooth. Reparative dentin that is formed by the nature healing process of tooth is similar (but not identical) to physiological dentin that is formed during tooth development. Unlike the Physiological dentin, reparative dentin is only a thin layer of poorly organized mineralized bridge secreted by odontoblast-like cells exhibiting impaired differentiation. However, the underlying mechanisms for the impaired differentiation of odontoblasts during reparative dentinogensis are not fully understood. The summer research project will include examining the inhibitory role of RUNX2, a transcription factor with a well-established stage-specific role in odontoblast differentiation in reparative dentinogensis. HRP SU22-21
Dr. Marmar Moussa  Department of Medicine (Neag Comprehensive Cancer Center) Bioinformatics and Genomics advances, especially in cell capturing technologies as well as whole-genome and whole-transcriptome amplification are allowing for the sequencing of the minute amounts of DNA and RNA from a single cell. An unprecedented window into the extent and nature of genomic and transcriptomic heterogeneity under both normal and disease conditions is now open and single-cell approaches stand poised to revolutionize our capability to understand the scale of genomic, epigenomic, and transcriptomic diversity that occurs during the lifetime of an individual organism. In our lab, we study the major technological and biological breakthroughs achieved in this field and actively address the remaining challenges in computational methods and algorithms development related to the field of single cell genomics. Development of computational methods using data science programming languages like R and Python will be the focus of this project. Undergrad students will get acquainted with bioinformatics tools and data science programming languages as well as participate in testing advanced computational methods and data collection and wrangling. HRP SU22-22
Dr. Lakshmi Nair Department of Orthopedic Surgery The goal of the project is to develop bioactive nano-lipid formulation for treating musculoskeletal pain. The study involves preparing the formulation with different molar ratios of the lipids and study how this change affects the shape and structure of the nano-lipid structures. Also involves developing purification methods for the formulation and study the impact of the purification methods on the morphology of the lipids. HRP SU22-23
Dr. Carla Rash  Department of Medicine We have two active opportunities that the student can choose from. The first is a NIH funded randomized clinical trial of interventions for employment seeking in persons living with HIV. The second project is a SAMHSA funded project focused on medication assisted treatment for individuals with opioid use disorder. Both projects involve opportunities to conduct interviews with patients and both research sites are located in Hartford. HRP SU22-24
Dr. Tannin Schmidt Department of Biomedical Engineering Lubricin is a protein present in synovial fluid that maintains cartilage and joint health through its lubricating and anti-inflammatory properties. Our lab recently discovered lubricin also plays a role in bone health, in its ability to inhibit inflammatory bone loss. This research project aims to expand on this discovery and understand how lubricin helps prevent bone loss, by evaluating its ability to interact with other regulating molecules and cell activity in bone remodeling and homeostasis. The project will employ in vitro biophysical, biomechanical, and biochemical assays to achieve this goal. HRP SU22-25
Dr. Henry Smilowitz  Department of Cell Biology Our laboratory, in combination with a small biotech company, pioneered the use of non-toxic high-Z nanoparticles, currently iodine nanoparticles, INPs, to powerfully enhance radiation therapy of tumors (INP-RT)--with an emphasis on the treatment of both primary and metastatic brain tumors and melanoma. Research projects this summer will include the use of orthotopic patient derived xenograft (PDX) models of primary and metastatic brain tumors as well as subcutaneous melanoma to study the efficacy of the INPs in mouse tumors that approximate the tumors in patients. Research projects on nanoparticle (NP) tumor loading (MicroCT, NP microdistribution (Confocal fluorescence microscopy), NP efficacy (in vivo radiation therapy), and synergy with other therapies such as chemotherapy and immunotherapy. HRP SU22-26
Dr. Timothy Spellman Department of Neuroscience How do higher-order brain circuits support flexible decision-making? This is an age-old question in neuroscience that can now be addressed in new ways, thanks to recent advances in tools for precisely controlling and monitoring neural activity. This project will use optogenetic methods, high-throughput rodent behavioral tasks, and cutting-edge computational tools to map the neuromodulatory cell types in the prefrontal cortex responsible for cognitive flexibility. Students will take responsibility for helping fine-tune, execute, and analyze the data from this project. HRP SU22-27
Dr. Melanie Tran  Department of Medicine Renal fibrosis is the pathophysiological hallmark of chronic kidney disease, which is characterized by fibroblast activation and dysregulation of the extracellular matrix. However, the mechanisms implicated in renal interstitial fibrosis are not well understood. This research project aims to investigate signaling pathways that regulate fibroblast activation and renal fibrosis. The student will gain extensive experience in cell culture, immunofluorescence, immunohistochemistry, Western blot and animal models of kidney disease. HRP SU22-28
Dr. Rajkumar Verma  Department of Neuroscience We study purinergic receptors P2X4 ( a receptor for ATP) in stroke pathology. In brief, following a stroke, a burst of ATP is released from dying brain cells which further activates both neurons and microglial P2X4R to mediate fast excitatory neurotransmission via cation influx. Excessive activation of P2X4R causes the release of several pro-inflammatory cytokines at the early time point of ischemic injury. However, contrary to the effects of acute activation, chronic inhibition or absence of this receptor may impair stroke recovery. Therefore, given this dual temporal role of P2X4R in ischemic injury, we are systematically exploring for its therapeutic potential in post-stroke recovery. HRP SU22-29
Dr. Penghua Wang  Department of Immunology This project will identify and functionally characterize the E3 ligases that are essential for SARS-CoV-2 (the virus that causes COVID-19) replication. We will apply CRISPR-Cas9 technology to generate gene knockout cells, examine if SARS-CoV-2 replication is impaired in gene-deleted cells when compared to gene-sufficient cells. We will then understand how the candidate genes help virus replicate, i.e., molecular mechanism of action. Significance: The candidate host genes could be potential antiviral drug targets. HRP SU22-30
Dr. Yanlin Wang  Department of Medicine We are interested in the cellular and molecular mechanisms of kidney injury and fibrosis. A major pathological feature of kidney disease is inflammation. Currently, we are examining the role of PU.1, a transcription factor, in macrophage activation and polarization. HRP SU22-31
Dr. Zhao-Wen Wang  Department of Neuroscience We recently discovered that melatonin promotes sleep in the nematode C. elegans by activating the SLO-1 potassium channel through a specific melatonin receptor, PCDR-1 (Niu et al., PNAS 117: 25128-25137, 2020). This project is to identify the neurons where PCDR-1 and SLO-1 act to regulate sleep by analyzing activities of neurons in the C. elegans sleep neural circuit using a genetically encoded calcium indicator, and by correlating neuronal activities with worm sleep behavior. HRP SU22-32
Dr. Yi Wu  Center for Cell Analysis and Modeling The input of signal transduction is not limited to chemical cues. The mechanical properties of the cellular microenvironment, such as rigidity, are also sensed by the cells and lead to both transient cellular dynamics and persistent gene regulation. The phenomenon is called mechanotransduction which attracts great interest in research in recent years yet remains poorly understood. The project will use a novel fluorescent reporter and live-cell imaging to investigate how cells convert the mechanical cues to chemical signaling in space and time. HRP SU22-33
Dr. Yulan Xiong  Department of Neuroscience The research opportunity available for the summer is to identify the new molecules involved in Parkinson's disease (PD) pathogenesis by performing genome-wide genetic screenings using single cell organism budding yeast. After identifying the new molecules, we will initialize the characterization of the functions of these new genes by performing a series of biochemistry studies (e.g. molecular cloning, western blotting, Co- immunoprecipitation, Confocal microscopy etc) using human cell and primary neuronal cultures. HRP SU22-34
Dr. Ping Yan  Center for Cell Analysis and Modeling There is a research opportunity on developing new voltage sensitive dyes, which are used for imaging membrane potentials. The student will have broad hands-on experience on the synthesis of organic compounds, and test the newly developed voltage sensitive dyes using spectroscopic and microscopic methods, on model cell membrane, and even on real cells (in collaboration with Dr. Loew). HRP SU22-35
Dr. Dong Zhou Department of Medicine, Division of Nephrology This summer project proposes to investigate how Calponin 2 (CNN2) determines the prognosis of acute kidney injury (AKI). CNN2 is an actin filament-associated regulatory protein that plays a central role in numerous fundamental biological processes, including cell proliferation, motility, and adhesion to substrates or others cells. Emerging evidence suggests that cell mechanics can have direct, non-transcriptional influences on cell metabolism. AKI is a refractory clinical syndrome associated with extremely high mortality and morbidity. However, very few studies pay attention to the metabolic changes after AKI. Therefore, in this summer project, we will investigate the role of CNN2 in AKI onset and progression from this new perspective, cell metabolism. The applicant will need to perform in vivo, in vitro, ex vivo experiments and learn the skills of bioinformatic analysis for the generated proteomics data for this project. HRP SU22-36

Summer 2021 Research Opportunities

Summer 2021 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Corey Acker Department of Cell Biology The student will support testing of new voltage-sensitive dyes using cell culture assays. Custom fluorescence microscopy equipment is used along with patch clamp electrophysiology to control and monitor the voltage inside cells, including human stem cell derived heart cells, by recording voltage changes optically as well as electrically. MATLAB is used for all aspects, from hardware control to data analysis. SU21-1
Dr. Tom Agresta Center for Quantitative Medicine Seeking a student researcher to participate in research and development of user interface designs for a mobile application to reconcile medications. The student will participate in qualitative and quantitative research on end user display options, and implement user interface design by modifying an existing product. The student will participate in work with end users to identify requirements, develop and share mock interfaces, and host focus groups around features that are important for end users of a medication reconciliation application. SU21-2
Dr. Srdjan Antic Department of Neuroscience In Alzheimer’s disease (AD), the loss of synaptic function is suspected before accumulation of characteristic amyloid plaques in cerebral cortex, and long before a patient presents with symptoms. In this project, we wanted to quantify the changes in synaptic function that occurs in AD model mice. Identifying these changes, will help towards development of earlier therapeutic interventions and diagnosis. Experiments include optical imaging of brain slices with synaptic stimulation paradigms, histological treatments of fixed brain slices for measuring accumulation of amyloid plaques, and fluorescent and confocal microscopy. Note that students will not work with live animals or have any contact with live animals. SU21-3
Dr. Michael Blinov Center for Cell Analysis and Modeling Mathematical modeling of biological processes is important to gain understanding of the underlying biological mechanisms and predict dynamics and outcomes of experiments and medical interventions. Mathematical models describe interactions among components of biological systems. Models are implemented and simulated in the Virtual Cell (http://vcell.org) software using GUI. We will develop a set of small models (ModelBricks, http://modelbricks.org) that serve as building blocks for larger models. A related project is connecting them to ImageJ software. SU21-4
Dr. Margaret Briggs-Gowan Psychiatry This coming summer, our lab will be conducting multiple studies to learn about how stress and violence affect infants and young children. The first study is an NIMH-funded study investigating the effects of domestic violence on young children, ages 4-6 years. The summer intern will have the opportunity to assist with study activities which include developmental testing, computerized activities that assess children's processing of facial and auditory stimuli, puppet interviews, and in-depth interviews with mothers about family life and the child's development, posttraumatic stress, and well-being. Psychophysiological data are acquired during visits including event-related brain potentials. The second study will be investigating how stress associated with the pandemic is affecting infants born during the pandemic. This study will involve multiple methods, including surveys, interviews, videotaped assessments, DNA samples and potentially MRI. The summer intern will be an integral member of our study team and will have ample opportunity to get hands on research experience in these studies. SU21-5
Dr. Doug Brugge Public Health Sciences We are interested in communicating the risk from air pollution near highways to people who live next to them. A summer intern would help us with our health communication efforts. Last summer our intern helped with evaluating an infographic we developed. Similar work is anticipated this next summer. SU21-6
Dr. Stephen Crocker Department of Neuroscience Extracellular vesicles, also referred to as “exosomes” are a primordial form of intercellular communication. We have recently reported that extracellular vesicles isolated from blood plasma when administered to mice with neuroinflammation triggers a shift in their adaptive immune response and a spontaneous clinical disease phenotype more closely resembling human multiple sclerosis (Willis et al. 2019 Proc.Natl.Acad.Sci, 116:10488-493). This project will delve into the characterization of the autoimmune T cells initiated in this model using RNAseq, TCR profiling and flow cytometry techniques. SU21-7
Dr. Caroline Dealy Reconstructive Sciences/Cell Biology/Orthopedic Surgery/Biomedical Engineering Post-Traumatic Osteoarthritis (PT-OA) is a severe and rapidly progressing joint degeneration that follows acute traumatic injury to the articular cartilage of the knee or ankle. Osteochondral allografting is a surgical approach that attempts to delay PT-OA onset by replacing the damaged articular cartilage with healthy articular cartilage obtained from a donor tissue bank. A challenge that limits the effectiveness of this approach is lack of seamless healing at the donor-host cartilage interface. In animal studies, we have identified a growth factor that stimulates cartilage repair potential by progenitor cells present in articular cartilage. The long-term goal of this project is to use this growth factor to develop a clinically-feasible approach to achieve seamless healing between the implanted donor graft cartilage and the patient’s own articular cartilage. SU21-8
Dr. Richelle deMayo Department of Pediatrics Research in the Division of Biomedical Informatics involve translational research projects, applying evidence-based health information technologies to improve health care processes and outcomes. The student would select a project from the following: - evaluation of aspects of telemedicine (provider wellbeing, telehealth equity, provider training effectiveness) - evaluation of artificial intelligence/machine learning for provider documentation efficiency, - evaluation of predictive models to better identify septic pediatric patients, - evaluation of pediatric-specific medication alerts to improve safety and reduce alert fatigue, - evaluation of the use of self-service analytics tools to support quality improvement efforts. – evaluation of a tool to reduce ICU-related delirium in pediatric patients. SU21-9
Dr. Alix Deymier Department of Biomedical Engineering  We are interested in understanding the effects of a high acid diet on the musculoskeletal system. We have developed a mouse model of diet-induced acidosis and are now interested in examining how acid exposure affected the mouse rotator cuff. The selected student will learn to perform Raman spectroscopy, microCT, and mechanical testing on mouse shoulders to determine how acidic diet changed the structure, composition, and mechanics of the tissue. SU21-10
Dr. Zhichao Fan Department of Immunology Due to the limitation of the detection method, the dynamic changes of immune cells, such as neutrophils, monocytes, or lymphocytes, during inflammatory diseases is unclear. In vivo flow cytometry (IVFC) is a novel non-invasive diagnostic technique to quantify specific circulating cells in mice. By using IVFC, we can access the dynamics of immune cells during inflammatory diseases, such as LPS-induced systematic inflammation or lung inflammation. This project is suitable for undergraduate research since all experimental setups (mice, equipment, reagents, protocols) are established. SU21-11
Dr. Jennifer Garza Department of Medicine We are seeking students interested in an opportunity to work on a project related to physical activity among working pregnant women. During the summer semester, the student will primarily be responsible for data analysis of existing physical activity data and literature review. There may also be opportunities to work directly with participants on field data collection. SU21-12
Dr. Damion Grasso Department of Psychiatry The student may engage in human subjects research that broadly seeks to understand behavioral and biological factors that influence children's mental health, including posttraumatic stress disorder (PTSD) following exposure to trauma or violence. One study focuses on individual differences in how young children exposed to domestic violence respond to threat cues. Another emerging study examines the effects of the COVID-19 pandemic on mental health, including PTSD, in perinatal families. Research activities may include interacting with human research participants, data collection and management, guided readings, and guided data analysis. SU21-13
Dr. Carolyn Greene Department of Psychiatry I am seeking an undergraduate student intern to assist with the Parent and Child Emotions Study (PACES) a research study investigating emotion regulation among parents and children who have experienced trauma. The intern will assist with parent and child visits to our laboratory, where families will be completing questionnaires and engaging in dyadic tasks during which we will collect physiological and observational data. The intern will also assist with recruitment activities in the community, screening and scheduling subjects, administrative tasks, and data entry and analysis, and have the opportunity to utilize initial study data (or data from a prior study) to develop a poster or paper on emotion regulation and children’s functioning. The intern will receive training in relevant tasks and be expected to attend research team meetings. SU21-14
Dr. Arthur Gunzl Department of Genetics and Genome Sciences Our research is on gene expression factors and mechanisms in the lethal human parasite and unicellular eukaryote Trypanosoma brucei (we work with a strain that is not infectious for humans). We investigate transcription and RNA splicing of pre-mRNA, processes that deviate substantially between trypanosomes and the human system. Current projects that are open for participation focus on a cyclin-dependent kinase (CDK) complex that is essential for protein coding gene expression and parasite viability as well as on the basal function of only two introns the parasite has retained for hundreds of million years of evolutionary time. Participation in either project will comprise learning of cell culture techniques, molecular cloning and a variety of biochemical, genetic and imaging techniques. While the focus will be on wet lab experience, there may be an opportunity for the application of next generation sequencing. On the theory side, there will be opportunities to understand the intricacy of the host-pathogen interaction. SU21-15
Dr. Rosa Guzzo Department of Neuroscience The focus of my research is understanding the epigenetic regulation in the development of the endochondral skeleton. We have identified a novel role for Dot1L, a chromatin modifier, in cartilage development and joint morphogenesis. Mice lacking expression of this gene in embryonic development exhibit a unique skeletal dysplasia phenotype. Research projects in my laboratory will utilize novel animal models systems of genetic loss of Dot1L function, primary cell cultures, differentiation assays, transcriptome analysis and chromatin assays to define the developmental and epigenetic contribution of Dot1L in embryonic and postnatal development. SU21-16
Dr. James Li Department of Genetics and Genome Sciences We study how the brain develops and how abnormal development contributes to brain disorders. We are using a number of approaches, including single-cell genomics and epigenomics, molecular biology, and experiments in stem cells and animal models (mouse and chick), to study the generation of various neurons and the assembly of neural circuitry, particularly in the cerebellum. Our multidisciplinary study will provide students with ample opportunities to explore different research approaches and identify a project that aligns with the individual’s skill, interest, and career goal. SU21-17
Dr. Changchun Liu Department of Biomedical Engineering Molecular diagnostics plays a critical role in rapid detection and identification of infectious diseases (e.g., COVID-19) (Ding, X. et al., Nature Communications, 2020, 11, 4711). In this summer project, we will develop microfluidics technology for nucleic acid detection. The microfluidic device will be fabricated by using 3D printing technology. Please find more detail on our current research through the website: https://smds.engr.uconn.edu/. SU21-18
Dr. Leslie Loew Center for Cell Analysis and Modeling The lab is using computational methods to understand cell function. The project will involve learning how to use a cell modeling software tool that was developed in my lab called SpringSaLaD (https://vcell.org/ssalad). You will use it to understand the molecular and cellular principles controlling the assembly of molecular machines composed of many individual molecules containing multiple binding sites. If you are interested in computer programming, there will also be the opportunity to work on improving SpringSaLaD. SU21-19
Dr. Pedro Mendes Center for Cell Analysis and Modeling Investigate the presence of chaotic dynamics in a set of differential equation-based models of biological systems. The student will use the software COPASI, and its cloud-based version Cloud-COPASI, to apply different global optimization algorithms in order to search for the existence of aperiodic behavior (chaos) in those models. The project will allow the student to learn about mathematical models of biological systems, computational systems biology, and simulation using high-performance computing. SU21-20
Dr. Wendy Mok Molecular Biology and Biophysics Our lab is interested in the problem of antibiotic treatment failure. In particular, we are investigating the survival strategies of bacterial persisters, which are rare cells within genetically clonal populations that can withstand lethal doses of antibiotics without acquiring mutations. Using microbiological, genetic, and analytical techniques, the student will explore the impact of environmental factors— including those stemming from microbe-microbe interactions—on the response and tolerance of Escherichia coli or Staphylococcus aureus persisters to antibiotics targeting different cellular processes. SU21-21
Dr. Siddika Mulchan Department of Pediatrics This project seeks to examine the feasibility and efficacy of a health care provider-focused intervention to reduce implicit racial bias toward youth with sickle cell disease (SCD). Specific aims are to: 1) examine the feasibility of an intervention incorporating individuation and perspective-taking targeting health care provider implicit racial bias toward youth with SCD, 2) examine the efficacy of the intervention on reducing health care provider implicit racial bias in pediatric SCD, and 3) examine whether the intervention contributes to less biased decision-making in pediatric SCD clinical care. Opportunities to assist with the development and validation of computer-based vignettes with artificial intelligence (AI) interface to ensure measures to assess implicit racial bias are scientifically-valid. SU21-22
Dr. Douglas Oliver Department of Neuroscience The student project will be to assist in the testing of an electrophysiological test for tinnitus in human subjects. The student will assist in the enrollment and scheduling of new subjects and in the actual testing. This is an electrophysiological test where brain activity is ecorded from scalp electrodes while the subjects are listening to sound. It is similar to that used for testing newborn babies in hospital if they are suspected of having a hearing loss. It time permits, the student may participate in the behavioral training of mice and their electrophysiological testing. SU21-23
Dr. Carla Rash Department of Medicine Our lab uses clinical trials to investigate a behavioral treatment called contingency management to motivate behavior change. Two of our current NIH-funded studies examine how to help people persevere in the long and often difficult process of getting employment: one is with unemployed persons living with HIV/AIDS and the other is unemployed persons with hazardous alcohol use. SU21-24
Dr. Blanka Rogina Department of Genetics and Genomic Sciences Indy (I'm not dead yet) encodes a plasma membrane citrate transporter predominantly expressed in fly metabolic tissues: the midgut, fat body and oenocytes. We have shown that organism-wide reduction in Indy activity extends fly health and longevity by altering energy metabolism. We are performing experiments to determine the effects of tissue-specific INDY reduction on fly health (mobility, stress resistance, metabolism) and longevity. Our preliminary data indicate that gut-specific Indy reduction in flies recapitulates many health benefits found in Indy hypomorphs including longer lifespan. Proposed experiments will address contributions of tissues-specific Indy reduction and shed light on the mechanism by which Indy reduction increase fly health and longevity non-autonomously. SU21-25
Dr. Melissa Santos Department of Pediatrics Connecticut Children's has launched our health disparities research group. This group has several projects undergoing all under the umbrella of making sure all families have the same access to care and are included in relevant clinical research being conducted at our institution. Students will work to extract data from our electronic health record in order to support these projects. SU21-26
Dr. Henry Smilowitz Department of Cell Biology Our lab in collaboration with a small biotech company pioneered the used of high-Z nanoparticles to enhance radiation therapy of tumors--with an emphasis on brain tumors. We have shown that novel iodine nanoparticles (INPs) enhance radiation therapy of advanced gliomas and metastatic breast tumors growing in the brains of mice. We wish to extend our studies to patient derived xenograft (PDX) tumors growing in the brains of mice. Our focus will be the ability of targeted and non-targeted nanoparticles to load selected PDX tumor models with defined characteristics. SU21-27
Dr. Rajkumar Verma Department of Neuroscience In one of the main projects of the lab, we study role of purinergic receptors P2X4 (a receptor for ATP) in stroke pathology. In brief, following a stroke, a burst of ATP is released from dying brain cells which further activates both neurons and microglial P2X4R to mediate fast excitatory neurotransmission via cation influx. Excessive activation of P2X4R causes the release of several pro-inflammatory cytokines at the early time point of ischemic injury. However, contrary to the effects of acute activation, chronic inhibition or absence of this receptor may impair stroke recovery. Therefore, given this dual temporal role of P2X4R in ischemic injury, we are systematically exploring for its therapeutic potential in post-stroke recovery. SU21-28
Dr. Yanlin Wang Department of Medicine Several research projects are available to summer research students. The specific project will be tailored to student’s interest. Our lab are interested in the pathogenesis of chronic kidney disease. A key pathologic feature of chronic kidney disease is renal fibrosis, which is characterized by fibroblast activation and excessive production and deposition of extracellular matrix. We are investigating several signaling pathways that regulate fibroblast activation into myofibroblasts. Specifically, we are studying TGF-beta/Smad signaling and JAK3/STAT6 signaling in fibroblast activation.   SU21-29
Dr. Yi Wu Center for Cell Analysis and Modeling Optogenetics has become a powerful tool in studying signaling transduction, thanks to its precise control at the speed of light and the spatial resolution of the diffraction limit. Specifically, the LOV domain (Wu et al., Nature 2009) can exert precise control over a signaling protein in its subcellular localization, protein-protein interaction, and allosteric regulation of enzymatic activity. We are looking for a motivated student to conduct protein engineering studies and learn with us how to control protein kinases or any other signaling proteins with light. SU21-30
Dr. Ming Xu Center on Aging My lab is trying to find novel interventions to slow down the aging process, and alleviate a number of diseases as a group. My recent work has shown several drugs can extend lifespan and maintain physical function in aged mice. The project here is to find new drugs for delaying aging using primary human cells and mice. Student(s) will gain extensive experience on cell culture, mouse studies, and overall aging research. SU21-31
Dr. Ping Yan  Center for Cell Analysis and Modeling There is a research opportunity on developing new voltage sensitive dyes, which are used for imaging membrane potentials. The student will have broad hands-on experience on the synthesis of organic compounds, and test the newly developed voltage sensitive dyes using spectroscopic and microscopic methods, on model cell membrane, and even on real cells (in collaboration with Dr. Loew). SU21-32
Dr. Daniel Youngstrom Department of Orthopedic Surgery Multiple projects are available related to musculoskeletal stem cell differentiation in the contexts of development/regeneration of 1) craniofacial bone and 2) intervertebral disc. Depending on the project, the student will gain skills in some or all of the following: wet lab cell/molecular biological techniques, histological workflows, imaging modalities including fluorescence microscopy and x-ray microtomography, zebrafish husbandry and surgical models, and/or mammalian cell culture. The student will be required to keep accurate experimental notes, regularly report on progress in small group settings, and ultimately function with increasing independence in a small and dynamic team. SU21-33
Dr. Yanjiao Zhou Department of Medicine My lab is a computational biology lab. We are interested in understanding interactions of airway and gut microbiome with mucosal immunity in asthma and aging. The technique we use includes anaerobic culture, molecular biology, and multi-OMICS technology. The students will have the opportunity to learn wet bench techniques and bioinformatics, depending on their interests. SU21-34

Summer 2020 Research Opportunities

Summer 2020 Research Opportunities

Summer 2020 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Corey Acker Department of Cell Biology The student will support testing of new voltage-sensitive dyes using cell culture assays. Custom fluorescence microscopy equipment is used along with patch clamp electrophysiology to control and monitor the voltage inside cells, including human stem cell derived heart cells, by recording voltage changes optically as well as electrically. MATLAB is used for all aspects, from hardware control to data analysis. SU20-1
Dr. Byoung-Il Bae  Department of Neuroscience The student will study the neurodevelopmental basis of autism spectrum disorder. She/he will characterize rare variants of the neurodevelopmental gene ASPM, which have been implicated in autism. She/he will generate the mutant constructs by site directed mutagenesis, and evaluate their effects on the protein levels of ASPM, the Wnt/beta-catenin signaling pathway, and neural progenitor cell proliferation. Successful completion of this project will elucidate how young patients with autism have larger than normal head circumferences ("macrocephaly"), and how abnormal neurodevelopment alters neural circuits for social behaviors. SU20-2
Dr. Jean-Denis Beaudoin Department of Genetics and Genome Sciences The trainee will have the opportunity to use CRISPR technologies to generate zebrafish loss of function mutant of RNA helicases. This project includes bioinformatics search of RNA helicase candidates, design of CRISPR strategies, injection of CRISPRs in zebrafish embryos, genotyping fish to find mutant alleles and look for developmental and molecular defects in loss of function mutants. Depending on the trainee's interest, there is also a possibility of using cell lines to study translation regulation using transfection and massive parallel reporter assays (measuring the regulatory activity of thousands of sequences in a single experiment using high throughput sequencing). SU20-3
Dr. Michael Blinov Center for Cell Analysis and Modeling Mathematical modeling of biological processes is important to gain understanding of the underlying biological mechanisms and predict dynamics and outcomes of experiments and medical interventions. Mathematical models describe interactions among components of biological systems. Models are implemented and simulated in the Virtual Cell (http://vcell.org) software using GUI. We will develop a set of small models (ModelBricks, http://modelbricks.org) that serve as building blocks for larger models. SU20-4
Dr. Margaret J. Briggs-Gowan Department of Psychiatry The Adaptation and Resilience in Childhood Study is an NIMH-funded study investigating the effects of domestic violence on young children, ages 4-6 years. The summer intern will have the opportunity to assist with study activities which include developmental testing, computerized activities that assess children's processing of facial stimuli, puppet interviews, and in-depth interviews with mothers about family life and the child's development, postraumatic stress, and well-being. Psychophysiological data are acquired during visits, including heart rate, skin conductance and event-related potentials. The summer intern will be an integral part of the study team and will have ample opportunity to get hands on research experience. SU20-5
Dr. Caroline Dealy Departments of Reconstructive Sciences, Biomedical Engineering, Orthopedic Surgery and Cell Biology The overall goal of this project is to develop a clinically-relevant approach to improve articular cartilage repair. In animal studies, we have identified a growth factor that stimulates cartilage repair potential by tissue-resident progenitor cells present in articular cartilage. This summer project will take the first translational step towards our goal by evaluating the effects of the pro-regenerative growth factor on progenitor cells in human articular cartilage. The project will identify the optimal dose and duration of growth factor treatment that maximally stimulates progenitor cell repair potential. Techniques will include tissue culture, histology, PCR, immunohistochemistry, confocal microscopy and digital imaging. SU20-6
Dr. Jennifer Garza Department of Medicine, Division of Occupational and Environmental Medicine We are seeking students interested in a summer research opportunity to join the UConn Study on Aging, Musculoskeletal Health, and Retirement (UConn-SAM) team. For their summer projects, students will measure work and out-of-work activity patterns of UConn-SAM participants with and without eldercare responsibilities. The opportunity includes field work, recruitment and interaction with study participants, and analysis of work and out-of-work activity data. Students will test the hypothesis that individuals with eldercare responsibilities will have different work and out-of-work activity patterns compared to those without eldercare responsibilities. SU20-7
Dr. Damion Grasso  Department of Psychiatry Student effort would involve interacting with human research participants and participating in data collection/management on two primary projects conducted at our Family Adversity and Resilience Research Program in West Hartford. The first is a follow-up component of an NICHD funded study examining the intergenerational transmission of trauma and stress in mothers and their infants. Research activities involve a 3-hour visit in which mother and infant participate in a laboratory stress paradigm and physiological data are collected to measures infant stress reactivity. The second is an NIMH funded study examining biological and behavioral indicators of stress reactivity to explain the relationship between early violence exposure and mental health problems in 4- to 6-year-old children. Research activities involve a 4-hour visit with parent and child specific assessments and mother-child tasks. SU20-8
Dr. Carolyn Greene Department of Psychiatry I am seeking an undergraduate student intern to assist with the Parent and Child Emotions Study (PACES) a research study investigating emotion regulation among parents and children who have experienced trauma. The intern will assist with parent and child visits to our laboratory, where families will be completing questionnaires and engaging in dyadic tasks during which we will collect physiological and observational data. The intern will also assist with recruitment activities in the community, screening and scheduling subjects, administrative tasks, and data entry and analysis, and have the opportunity to utilize data from a prior study to develop a poster or paper on emotion regulation and children’s functioning. The intern will receive training in relevant tasks and be expected to attend research team meetings. SU20-9
Dr. Kshitiz Department of Biomedical Engineering We have established a fascinating connection between pregnancy and cancer metastasis, fundamentally changing our view of how and why cancer becomes malignant (see our paper in Nature Evolution: https://rdcu.be/bZk0D). This discovery has opened up a new and important field to investigate cancer metastasis, how it starts, why it starts, and suggest methods to control cancer invasion. We are listing a series of very interesting projects for HRP students, and I am sure you will like at least one of them, and will get to work on a variety of techniques. These include: 1. Understanding the mechanics of how cancer invades into the surrounding tissue: involves microscopy, image analysis, traction force measurements etc. 2. Understanding the metabolism of cancer invasion: microscopy, metabolomics, lots of cool assays, bioinformatics, data analysis. 3. The evolutionary basis different levels of malignancy in mammals: dealing with farm animal tissues, histology, bioinformatics. 4. Looking at how fibrosis occurs in different tissues: nanoengineering, microscopy, image analysis, force generation analysis, and basic biochemistry.Students in our group have a high probability to be part of  publications if they contribute. SU20-10
Dr. Liisa Kuhn  Department of Biomedical Engineering This project will involve using 3D scans of mastectomy patients and 3D printing to create a personalized breast prosthesis that can be worn externally to restore symmetry and aesthetics to breast cancer patients. The project requires learning how to use the scanning software skanect and the program meshmixer and solidworks or autocad and a 3D printer. The challenge will be to optimize the print parameters and design of the part to make the part print in a half a day or less while offering structural support and comfort to the patient. SU20-11
Dr. Sangamesh Kumbar Orthopedic Surgery The student will be involved in the design, development, and fabrication of polymeric micro-nano structures for tissue regeneration and drug delivery. These structures will be characterized for their physicochemical and biological properties using in vitro and in vivo test models. Specifically, the student will work on conducting drug release and accessing the in vitro cell response to the released drug. The student will be introduced to protocols to conduct experiments, data acquisition, analysis, oral presentations and report writing. SU20-12
Dr. Changchun Liu Department of Biomedical Engineering Nucleic acid-based molecular detection plays a critical role in rapid diagnostics and prompt treatment of infectious diseases. In this summer project, we will design, fabricate and test microfluidic diagnostic device and portable detection system for point of care diagnostics. The device and system will be fabricated by 3D printing technology. Please find more detail on our current research through the website: https://smds.engr.uconn.edu/. SU20-13
Dr. Leslie Loew Berlin Center for Cell Analysis and Modeling The lab is using computational methods to understand cell function. The project will involve learning how to use a cell modeling software tool that was developed in my lab called SpringSaLaD (https://vcell.org/ssalad). You will use it to understand the molecular and cellular principles controlling the assembly of molecular machines composed of many individual molecules containing multiple binding sites. If you are interested in computer programming, there will also be the opportunity to work on improving SpringSaLaD. SU20-14
Dr. Kazuya Machida Department of Genetics and Genome Sciences The goal for the summer is to develop a new single cell protein binding assay using advanced flow cytometry technologies. The student will a) construct a panel of labeled protein domain probes, b) validate the probes in biochemical and imaging analyses, c) and optimize the assay conditions using human lymphocytes. Together, these experiences enhance the student's abilities for future careers. SU20-15
Dr. Kevin Manning Department of Psychiatry This is a clinical research opportunity for students interested in geriatric psychiatry or clinical neuroscience. We (a collaborative group of psychologists, psychiatrists, and neuroscientists) have an ongoing clinical trial aimed at understanding whether cognitive fitness (computerized brain games) improves both depression symptoms and cognitive functioning in a sample of older adults with treatment resistant major depression. The student will learn about the day to day operations of clinical neuroscience research by learning to administer behavioral tests and measures / entering in data to a database / and observing MRI assessments and psychiatric interviews. SU20-16
Dr. David Martinelli Department of Neuroscience Two different projects are underway in the lab, and the student could potentially choose either. The first regards the biochemistry of synaptic adhesion proteins, and is described well on the lab website https://health.uconn.edu/synapse/ . The second project is not described on the lab website, but involves the same set of proteins/genes, which happen to also be expressed in oligodendrocytes, the cells that make brain myelin. The project centers on understanding how myelin gets made, with the long term goal of developing a new treatment for multiple sclerosis. SU20-17
Dr. Bruce Mayer  Department of Genetics and Genome Sciences We have developed computational models that describe B cell receptor signaling, which is dysregulated in human leukemias such as Chronic Lymphocytic Leukemia (CLL). We have also found patterns of protein phosphorylation (which we term "SH2 profiles") in CLL patient samples that correlate with clinical outcomes such as disease progression. The goal of the proposed project is testing and validation of the computational model. This will involve both computational work, and biochemical studies using cell lines and patient tumor samples. SU20-18
Dr. Pedro Mendes  Center for Quantitative Medicine, Center for Cell Analysis and Modeling (Department of Cell Biology) A critical aspect in developing computational systems biology models is to estimate values for the parameters of a model based on experimental data. Our systems biology software COPASI (http://copasi.org) is one of the leading packages for parameter estimation, which is widely used in the literature (around 100 papers per year use it). However COPASI executes parameter estimation using optimization algorithms that run in serial mode and tcan be very slow. We aim to address this problem by implementing optimization algorithms, known as "island evolutionary algorithms", that can run in parallel making use of high-performance computing resources. This project will implement such an algorithm, to be written as a script in the R programming language and which will control COPASI through an existing API (https://github.com/jpahle/CoRC). This research project includes coding, debugging and benchmarking the algorithm using established test case problems. Finally we will apply it in an ongoing research project on genetic regulation by micro-RNAs. SU20-19
Dr. Masoud Nickaeen Center for Cell Analysis and Modeling (Department of Cell Biology) We will develop algorithms to numerically solve partial differential equations in domains with moving boundaries. We will implement these algorithms in computer programs and run simulations to evaluate their accuracy and validity. We will benchmark the utility of the new algorithms in the study of cellular processes that lead to or rely on the motion and deformation of the cells, their organelles or subcellular structures. SU20-20
Dr. Stefan Pinter  Department of Genetics and Genomic Sciences Project DescriptionOur lab studies genetic syndromes that change the expression levels of many genes residing on a single chromosome, for example Down syndrome (DS, trisomy 21) and Turner syndrome (TS, monosomy X). We have established human stem cell (iPSC) lines of these aneuploidies, along with isogenic euploid control lines, to model cellular phenotypes associated with these conditions. To correct the dosage of genes on chromosome X or 21, we use epigenetic (XIST RNA) and RNA-targeting CRISPR tools to study their developmental impact, and map cellular phenotypes back to specific mis-expressed genes. For example, we would like to learn how trisomic genes on chromosome 21 contribute to oxidative stress in DS neurons and astrocytes, and have built a reporter iPSC line to identify which genes would have to be targeted to restore a typical redox balance SU20-21
Dr. Tannin Schmidt Department of Biomedical Engineering Lubricin is multi functional protein, with both lubricating and anti inflammatory properties, that is present throughout the human body. In particular, lubricin is present in synovial fluid as well as tears, and is critical for both knee joint and ocular surface health. Recombinant human lubricin has been shown to be effective in treating osteoarthritis in preclinical models, and also improving signs and symptoms of dry eye disease in humans. This project will examine the regulation of lubricin biosynthesis by various relevant cell types, as well as further explore lubricin’s recently discovered anti-inflammatory properties. SU20-22
Dr. Henry Smilowitz Department of Cell Biology Project DescriptionOur laboratory in collaboration with a small biotech company, Nanoprobes, Inc. pioneered the use of heavy atom nanoparticles to enhance radiation therapy (RT) of tumors, with a focus on primary and metastatic brain tumors (Hainfeld et al., 2004). Our initial work used gold nanoparticles (Hainfeld et al. 2010, 2013). Our more recent work has been with well tolerated novel iodine nanoparticles (INPs) (Hainfeld et al., 2018, 2019). Current work in the lab is focused on 1. Improving INP-enhanced RT efficacy by tumor targeting of the INPs, 2. Studying the mechanism of INP-enhanced RT by A. Microlocalization of INPs in brain tumors, B. Quantification of double strand DNA breaks (DSBs) in tumor and non-tumor cells in the brain after RT. C. Combination of INP enhanced RT with chemotherapy, immunotherapy. Our lab also has a continuing interest in tumor dormancy and projects along those lines. For specific projects please come to talk with Dr. Smilowitz. For specific references to our published papers, please refer to our Cell Biology web site. SU20-23
Dr. Ali Tamayol Department of Biomedical Engineering Chronic wounds are major healthcare challenges that affect a noticeable number of people by exerting a severe financial burden and being the leading cause of limb amputation. Although challenging, healing rate can be enhanced by administration of therapies at the right time. The project in Laboratory for Innovative Microtechnologies & Biomechanics (LIMB) is focused on development of smart bandages for active monitoring of the wound environment using integrated biosensors followed by on‐demand drug delivery employing active and passive methods. The project is interdisciplinary combining biology, biomaterials, biochemistry, bioelectronics and biomechanics. SU20-24
Dr. Ephraim Trakhtenberg Department of Neuroscience We study how the brain develops and utilize gained knowledge to reverse-engineer regeneration of the brain tissue damaged by an injury or stroke. We employ a multidisciplinary approach spanning cutting edge genetics, epigenetics, bioinformatics, molecular biology, and gene therapy, which will provide a student with an opportunity to explore different approaches and select a project that aligns best with the individual’s career goals and interests. SU20-25
Dr. Paola Vera-Licona Center for Quantitative Medicine The student involved in this project will apply computational systems biology and bioinformatics tools to quantitatively study Acute Myeloid Leukemia maturation state interconversion in a clinically relevant in vivo model of differentiation therapy. Bioinformatics tools will include the use of the software package geneXplain (http://genexplain.com/) and some R packages to visualize and analyze RNA-seq data. In addition, the student will learn to use some Cytoscape apps (http://www.cytoscape.org/). SU20-26
Dr. Yi Wu Center for Analysis and Modeling (Department of Cell Biology) The student has an opportunity to participating in several projects related to mechanobiology. These projects overall hinge upon a newly developed, genetically-encoded biosensor from the lab for detecting mechanical forces in living cells. The exact project for the student can focus on calibrating force sensing modules in vitro, engineering force sensors for a specific protein, or measuring forces in live cell microscopy. SU20-27
Dr. Ping Yan Center for Cell Analysis and Modeling (Department of Cell Biology) There is a summer research opportunity for a chemistry student to synthesize new voltage sensitive dyes (VSDs). While mainly working on organic syntheses, the student will also measure the absorption and fluorescence spectra, test the sensitivities in artificial membranes, and possibly image action potential in real cells using newly synthesized VSDs (in collaboration with Dr. Loew). SU20-28
Dr. Riqiang Yan Department of Neuroscience Chemokines and cytokines play a role in a variety of degenerative diseases. This project will explore the role of a particular chemokine CXCL14 on Alzheimer's disease. Participants will be using imaging and biochemical techniques to localize and quantify CXCL14 in mouse models of Alzheimer's disease and Alzheimer's disease patient tissue. The effect of CXCL14 on cell migration into the brain will also be explored using culturing and live imaging techniques. SU20-29
Dr. Ji Yu Center for Cell Analysis and Modeling (Department of Cell Biology) Misregulation of protein phosphorylation is linked to important human diseases, particularly cancer. The goal of the project is to develop a microscopy method to analyze the phosphoproteome in a spatially resolved manner. We achieve this by combining the naturally existing phosphor-sensitive library of SH2 domains with a protease based multiplexing imaging scheme. Furthermore, the project also aims to establish and validate an imaging analysis pipeline that allows rigorous yet intuitive interpretation and visualization of the high-dimensional imaging data. SU20-30

Summer 2019 Research Opportunities

Summer 2019 Research Opportunities

Summer 2019 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Paola Bargagna-Mohan Neuroscience The cornea is the transparent tissue in the front of the eye, and its main function is to refract light. Injury/insult and diseases compromise corneal clarity, cause fibrosis and scarring, and ultimately blindness (4th leading cause of worldwide blindness). The corneal myofibroblasts are a differentiated mesenchymal cells known to cause fibrosis, and they are well studied. Now, we have discovered another type of corneal cells that actively contribute to corneal fibrosis: the corneal Schwann cells. This is the first time these cells are studied/explored in mouse corneas. The student researcher will contribute to characterizing these cells during wound healing and/or pathological fibrosis. SU19-1
Dr. Margaret Briggs-Gowan Psychiatry We are studying the effects of domestic violence on threat-reactivity and symptoms in young children, ages 4-6 years at our lab in West Hartford. Mothers and children are being evaluated in our laboratory with interviews, developmental testing, observation of mother-child interactions, computerized "games" and puppet interviews. We are also collecting psycho-physiologic measures, such as heart rate reactivity, skin conductance, and event-related brain potentials. Internship activities will include helping with study recruitment (e.g., distributing flyers to childcare centers), assisting with study visits, data preparation, and conducting a literature review on a related topic of their choosing. SU19-2
Dr. Jennifer Cavallari Community Medicine and Healthcare We are seeking a student to join the WorkTime research team studying the relationship between work hours and schedules and worker and family health. You will learn many aspects of field data collection among human participants, including consenting, survey administration, and data management and analysis, as we survey workers at their workplaces. You will also gain knowledge in Public Health, community-based-participatory research, and occupational health and skills in survey research methods, and data management and analysis. SU19-3
Dr. Bojun Chen Neuroscience This project is to identify genes that play important roles in controlling the reproductive lifespan in the nematode C. elegans. We have a C. elegans strain with a doubled reproductive lifespan. Using this strain, we plan to perform a genetic screen to isolate mutants that restore the normal reproductive lifespan. SU19-4
Dr. Alix Deymier Biomedical Engineering The study focuses on the effects of acidosis on the structure and mechanics of bones. Over the course of the spring semester, a number of mice will be given a diet that puts them into an acidodic state, meaning that their body pH will drop. After a few weeks of acidosis, the mice will be sacrificed. Students will learned how to collect and characterize the bones of these mice. This includes learning to dissect mice, perform mechanical tests, use Raman microscopy, analyze CT data, and perform histology. SU19-5
Dr. Mallika Ghosh Center for Vascular Biology Tunneling Nanotubes (TNTs) are specialized actin or microtubule-based membrane encased cell protrusions that hovering above the substratum, connecting two distant cells via one continuous structure for efficient cell-cell communication, transport of signaling molecules and cell organelles. Recent studies have reported TNT formation in a wide array of cells including neuronal, endothelial, as well as stem cells and immune cells. CD13, an adhesion molecule mediating cell-cell and cell-ECM adhesion, has been shown to regulate internalization of receptors to control downstream signaling. Studies from our laboratory have implicated a critical role for CD13 in regulating actin cytoskeletal organization and receptor recycling as well. Given CD13’s function in endocytosis and actin cytoskeletal organization, we chose to explore CD13’s role in the formation and function of TNTs. SU19-6
Dr. Carolyn Greene Psychiatry I am seeking an undergraduate student intern to assist with a research study investigating emotion regulation among parents and children who have experienced trauma. The intern will assist with parent and child visits to our laboratory, where families will be completing questionnaires and engaging in dyadic tasks during which we will collect physiological and observational data. The intern will also assist with recruitment activities and data entry, and have the opportunity to utilize data from a prior study to develop a poster or paper on emotion regulation and children's functioning. SU19-7
Dr. Arthur Gunzl Genetics & Genome Sciences Our research is on gene expression factors and mechanisms in the lethal human parasite and unicellular eukaryote Trypanosoma brucei (we work with a strain that is not infectious for humans). We investigate transcription and RNA splicing of pre-mRNA, processes that deviate substantially between trypanosomes and the human system. Current projects that are open for participation focus on a cyclin-dependent kinase (CDK) complex that is essential for protein coding gene expression and parasite viability as well as on the basal function of only two introns the parasite has retained for hundreds of million years of evolutionary time. Participation in either project will comprise learning of cell culture techniques, molecular cloning and a variety of biochemical, genetic and imaging techniques. While the focus will be on wet lab experience, there may be an opportunity for the application of next generation sequencing. On the theory side, there will be opportunities to understand the intricacy of the host-pathogen interaction. SU19-8
Dr. Reinhard Laubenbacher Center for Quantitative Medicine The student project is part of a larger program to develop a collection of algorithms for mathematical modeling and data analysis in biomedicine. The collection of algorithms is intended to be "crowd sourced," in the sense that algorithm developers from around the world can upload their own algorithms and link them with already posted algorithms into workflow pipelines. For this purpose, we are developing two tools that facilitate the packaging of algorithms and their computational environment into Docker containers, and connect them together through a drag-and-drop tool.The role of the student in the project is to contribute to the further development of these two tools. SU19-9
Dr. James Li Genetics and Genomics We study how the brain develops and how abnormal development contributes to brain disorders. We are currently using a number of approaches, including single-cell RNA sequencing, bioinformatics, molecular biology, and experiments in stem cells or animal models (mouse and chick), to study the generation of various cell types in the cerebellum. SU19-10
Dr. Changchun Liu  Biomedical Engineering Rapid and sensitive molecular diagnosis of diseases, including pathogens and cancer biomarkers, at the point-of-care is critical for prompt and appropriate therapeutic intervention. In this summer project, we will focus on developing microfluidic chip and biosensor for point of care molecular detection. The device will be fabricated by either 3D printing technology or wax printing technology. SU19-11
Dr. Kevin Manning  Psychiatry We are seeking students to assist in ongoing treatment studies of major depression in older adults. We have two ongoing studies: 1) a naturalistic study (where patients are taking standard antidepressants and are followed over time) and 2) a study that uses cognitive remediation (brain games) to improve cognitive functioning and change underlying neural activity (measured with fMRI) in patients. Interested students would help administer tasks and score/enter data into research databases. There are opportunities to use collected data for scientific conference presentations and papers (e.g., the naturalistic study has been ongoing for 5 years and has a sample of 140 older adults with major depression). SU19-12
Dr. David Martinelli Neuroscience Two different projects are underway in the lab, and the student could potentially choose either. The first regards the biochemistry of synaptic adhesion proteins, and is described well on the lab website https://health.uconn.edu/synapse/ . The second project is not described on the lab website, but involves the same set of proteins/genes, which happen to also be expressed in oligodendrocytes, the cells that make brain myelin. The project centers on understanding how myelin gets made, with the long term goal of developing a new treatment for multiple sclerosis. SU19-13
Dr. Royce Mohan Neuroscience The Mohan lab is interested in the study of retinal pathology using mouse models of experimental and genetically-induced neurodegenerative diseases. The student will be taught techniques such as, isolation of mouse eyes from sacrificed mice, cryosectioning of eyes, tissue staining using antibodies, and image acquisition by epifluorescence microscopy and western blot analysis. In certain cases, students may also advance to using live mice, where they will be taught mouse handling, perform injections to deliver anesthesia and drugs and terminal euthanasia. Some students do continue on the project in fall semester and may learn retinal injury. SU19-14
Dr. Lakshmi Nair Orthopedic Surgery The goal of the project is to develop bioactive polymeric biomaterials to decrease tissue inflammation and enhance healing. The work involves incorporating bioactive molecules to existing hydrogels, physico-chemical characterization of the hydrogels and in vitro cell culture studies to under the broader biological activity of the hydrogels. SU19-15
Dr. Douglas Oliver Neuroscience We are developing an electrophysiological test to demonstrate the presence or absence of tinnitus in animals and human subjects. During summer and fall 2019, a number of different projects are possible. 1) Use an active avoidance task and the inhibition of acoustic startle to test whether mice have tinnitus that is induced by exposure to a loud sound. Animals will be trained and tested before and after exposure to loud sound. 2) Use multichannel electrophysiological recordings from neurons in the midbrain auditory system of mice to study the changes in sound-evoked activity. 3) Develop the electrophysiological test for tinnitus using scalp recordings of sound-evoked responses in mice and eventually in human subjects. SU19-16
Dr. Carla Rash Medicine We have multiple NIH-funded R01s of clinical trials for addictions. All of these projects involve behavioral interventions, and most involve a specific intervention called contingency management. The specific project the student would be assigned would depend on the patch between their individual preferences and study needs at the time. Opportunities may include data entry, assisting with study auditing procedures, as well as direct contact with participants, conducting research interviews, and screening participants. SU19-17
Dr. Archana Sanjay Orthopaedic Surgery The overall objective of the project aims to identify and characterize novel regenerative adult stem/progenitor cells in the periosteum. Periosteum is a connective tissue that covers long bones as well as bones of the cranium, maxilla, and mandible, and it is required for efficient skeletal repair. Compromised, non-healing fractures pose a significant public health problem; understanding the molecular drivers of periosteum-mediated regeneration will help to develop new therapies to reduce the incidence of delayed union and nonunion in fracture healing of both long bones and the craniofacial region. Our laboratory has a longstanding interest in the periosteal response to fracture, as the periosteum is a critical reservoir for skeletal progenitors following injury7,8. We explored the possible presence of Lgr family members in the periosteal layer. Preliminary data show that Lgr6 is expressed in periosteal cells that proliferate at a bone fracture site, while there is undetectable expression of Lgr6 in periosteum of uninjured bones. SU19-18
Dr. Tannin Schmidt Biomedical Engineering Biomechanical characterization of cartilage tissue in synovial joints & the role lubricin/PRG4 plays in joint homestasis. Lubricin is a lubricating molecule originally discovered in the knee joint that is necessary for joint health. Intra-articular injections of recombinant human lubricin, which is also being developed to treat dry eye disease, have been shown to be effective preventing/delaying osteoarthritis disease progression. This project will cartilage integrity in synovial joints, via mechanical indentation, and the role lubricin plays in synovial joint integrity using various models. Biochemical analysis will also be employed to examine tissue composition of lubricin. SU19-19
Dr. Henry Smilowitz Cell Biology Our lab is studying heavy atom nanoparticles to enhance radiation therapy of tumors with an emphasis on brain tumor therapy. We also study the synergy of the combination of heavy atom nanoparticle radiation enhancement with other therapies such as chemotherapy and immunotherapy. We also study radiation therapy induced tumor dormancy. The student will take part in a project related to these lab interests. Topics of interest will include the relationship between the distribution of the nanoparticles and the efficacy of radiation enhancement, radiation enhancement and the efficacy of chemo- and immunotherapies, the effect of radiation dose and tumor dormancy. SU19-20
Dr. Rajkumar Verma Neuroscience Project 1: In this project we will investigate a novel drug target “Purinergic receptor P2X4” for therapeutic exploitation in stroke. The overall goal of this project is to determine if modulation of P2X4R signaling in myeloid cells is a viable therapy for stroke, working towards our long-term goal of developing and identifying target-based therapies for stroke. Project 2: Vascular dementia (VaD) is the second most common form of dementia after Alzheimer’s disease (AD). Although it is the most rapidly increasing disorder in the aging population, VaD remains under diagnosed, studied and treated. At the molecular level, VaD is characterized by key neuronal and dendro-synaptic changes resulting in dysfunction and cognitive deficits. Therefore, greater understanding of the pathophysiology at the molecular level is needed to identify novel vascular substrates of dementia. Our goal here is to identify key proteins involved in modification of brain pathology during progression of VaD. SU19-21
Dr. Penghua Wang Immunology To study the innate immune system, detection of viruses and initiation of innate antiviral immune responses, pathogenesis of viral arthritis. 2. To understand the mechanisms of immune evasion and modulation of cellular functions by flaviviral/alphaviral proteins. 3. To elucidate the impact of intestinal microbiome dynamics during early life on cell intrinsic antiviral immunity in intestinal epithelium. SU19-22
Dr. Yanlin Wang Medicine The summer research project is to examine the molecular signaling pathway that regulates fibroblast activation and monocyte-to-fibroblast transition. The student will utilize molecular, pharmacological and genetic approaches such as cell culture, Western blot, histology, gene knockout. SU19-23
Dr. Zhao-Wen Wang  Neuroscience We recently discovered that C. elegans AIPR-1, a homolog of human aryl hydrocarbon receptor-interacting protein, is a strong regulator of neurotransmitter release, and functions through stabilizing the calcium releasing channel ryanodine receptor in the endoplasmic reticulum membrane (Chen et al., Nat Commun 2017). We would like to identify proteins that are important to AIPR-1 function in vivo using a forward genetic approach. Specifically, the student will screen for mutant worms that suppress a behavioral phenotype caused by AIPR-1 deficiency. SU19-24
Dr. Zhao-Wen Wang  Neuroscience We need to add a dark-field tracking feature to an existing Matlab-based bright-field worm-tracking system so that a moving worm with selected neurons labeled by a red or green fluorescent protein may be tracked. In addition, we need to figure out approaches to quantify fluorescent signal intensity over time. SU19-25
Dr. Yi Wu Center for Cell Analysis and Modeling We are seeking a motivated undergraduate intern to join our research team and to help unravel the remaining mysteries in regulated secretion. The available projects involve using cutting-edge imaging technologies to observe individual secretory granules and their associated proteins in several model systems include neurons, pituitary cells and pancreatic beta cells. The student are expected to learn how to interrogate different cellular and signaling dynamics involved in secretion and hopefully gain new insights into the regulatory mechanisms. In addition to these biological projects, we are also welcome students who are interested in developing imaging tools in optogenetics and synthetic biology. SU19-26
Dr. Ming Xu Center on Aging My lab is trying to find novel interventions to slow down the aging process, and alleviate a number of diseases as a group. My recent work has shown several drugs can extend lifespan and maintain physical function in aged mice. The project here is to find new drugs for delaying aging using primary human cells and mice. Student(s) will gain extensive experience on cell culture, mouse studies, and overall aging research. SU19-27
Dr. Ping Yan Center for Cell Analysis and Modeling There is a research opportunity on developing new voltage sensitive dyes, which are used for imaging membrane potentials. The student will have broad hands-on experience on the synthesis of organic compounds, and test the newly developed voltage sensitive dyes using spectroscopic and microscopic methods, on model cell membrane, and even on real cells (in collaboration with Dr. Loew). SU19-28
Dr. Ji Yu Center for Cell Analysis and Modeling Two projects are available for summer students: 1. To design, synthesize and evaluate new fluorescent molecular probes that specifically label phosphotyrosines in cells. Perform various imaging assays using the fluorescent probes on model systems, and compare their relative performance. 2. To perform computational analyses of super-resolution imaging data using novel software developed in the lab. Evaluate the performance of the algorithm. SU19-29
Dr. Kristyn Zajac Calhoun Cardiology Center, Medicine I conduct research studies on behavioral interventions for promoting health outcomes, particularly related to addictive behaviors. I currently have three ongoing clinical trials testing: 1) a family-based therapy for Internet gaming disorder (aka Video Game Addiction); 2) a counseling intervention for young adults with substance use disorders and posttraumatic stress disorder and 3) a reinforcement intervention to promote HIV testing among at-risk women. Students will have the opportunity to be involved in one or more of these projects based on their interests. Student involvement can include: data entry and database management, assisting with ongoing trainings for our community partners (for the HIV study), assisting with advertising and recruitment of participants, participation in research team meetings, and use of existing databases to develop and answer research questions in the field of addictions. Direct contact with research participants may be available based on interest and student skill level. SU19-30
Dr. Yanjiao Zhou Medicine My lab is a computational biology lab. We are interested in understanding how the gut microbiome modulates local gut immunity and neuro-inflammation in multiple sclerosis, an autoimmune disease affecting central nervous system. The technique we use includes both anaerobic culture, molecular biology, and multi-OMICS technology. The students will have the opportunity to learn big data analysis and wet bench techniques, depending on their interests. SU19-31

Summer 2018 Research Opportunities

Summer 2018 Research Opportunities

Summer 2018 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Corey Acker Center for Cell Analysis and Modeling The student will assist with designing, assembling, and testing new microscopy methods. Modifications of an existing 2-photon microscope are planned as well as an epifluorescence microscope for studying cell signaling. SU18-1
Dr. Alix Deymier Biomedical Engineering The body is extremely sensitive to changes in pH and small disregulations can lead to pathologies like acidosis that cause tissue damage and osteoporosis. In the lab, you will be examining the structure and mechanics of bones that come from mice with acidosis. You will learn to perform dissections, do chemical and structural analysis and do mechanical testing. The goal is to determine how acidosis affects the skeleton and how we can minimize those effects. SU18-2
Dr. Naveed Hussain Pediatrics, Division of Neonatology Necrotizing enterocolitis is a devastating condition that affects premature infants. We are studying the epidemiology and risk factors associated with this condition using databases of NICU patients collected over the past 25 years at UConn Health and Connecticut Children’s Medical Center. Our aim is to develop prediction algorithms to find early warning signs (Neonatal Early Warning Signs-NEWS) that can be used clinically in identifying cases of NEC early enough to prevent their devastating consequences. SU18-3
Dr. Sivapriya Kailasan Vanaja Immunology The objective of the project will be to identify virulence factors of Enterohemorrhagic Escherichia coli (EHEC) that interact with the innate immune system. Specifically the project will include utilizing mutants of EHEC that lack virulence factors to infect macrophages and determine if the deficiency of these bacterial factors affect innate immune responses such as inflammasome activation. Inflammasome activation will be assessed by measuring cell death and cytokine responses following infection with the EHEC mutants. SU18-4
Dr. Insoo Kim Medicine In this project the student will participate in development of a wearable sensor for personal health monitoring - blood pressure, heart rate variability, and sleep monitoring. The nature of this project will require the student to develop engineering expertise in biomedical instrumentation, flexible sensor development, and digital signal processing. The student will be asked to participate in flexible sensor and system development, electronic circuit board building, and lab experiments with the sensor. SU18-5
Dr. Min Jung Kim Calhoun Cardiology Center In this project, the student will participate in developing an iOS app for tracking patient reported outcomes such as health related habits, heart failure symptoms, and quality of life in a patients with a diagnosis of heart failure. The nature of this project requires 1) to understand the importance of daily monitoring of biomarkers, activity levels, symptoms, and medication response for the patient struggling to manage chronic heart failure, 2) to implement the mobile application to support heart failure management strategies, and 3) to evaluate patient reported outcomes with mobile app. The student will be asked to develop the iOS app using X-Code software, and to analyze the collected data using statistical methodology. SU18-6
Dr. Liisa Kuhn Biomedical Engineering In this project the student will learn how to test biomaterials that might be used one day as a new bone graft. The biomaterials, also known as scaffolds, are placed in pieces of living bone that have been dissected from old mice and cultured in an incubator to allow the early steps of bone repair to occur in controlled conditions. The student will learn how to embed the biomaterials after they have been cultured and learn how to cut thin sections of them to look at the cell types under the microscope that migrate into the scaffolds with time. The goal of the summer research is to see how various compounds adsorbed on the biomaterial affect early inflammation processes and find the best ones that can help bones heal faster. SU18-7
Dr. Sangamesh Kumbar Orthopaedic Surgery & Biomedical Engineering Cell to cell communication plays a key role in the function and development of neural tissue. Neuronal cells communicate largely through electrical potentials. This summer’s research will focus on cell culture of neuronal and non-neuronal cells to study the underlying connection between electrical stimulation and neural phenotype development by mesenchymal stem cells. SU18-8
Dr. Reinhard Laubenbacher Center for Quantitative Medicine The Center for Quantitative Medicine at UConn Health is developing a software platform for computational algorithms related to medicine and healthcare. The platform is based on the relatively new technology of Docker software containers, which can be used to share environment-independent computational, mathematical, and statistical tools. The site algorun.org contains more information. Qualified undergraduate students will participate in the further development of this platform, learn novel software engineering techniques, become proficient in Docker applications, and participate in providing software solutions to problems in the health field. SU18-9
Dr. James Li Genetics and Genome Sciences We study how the brain develops and how abnormal development contributes to brain disorders. We are currently using a number of approaches, including single-cell RNA sequencing, bioinformatics, molecular biology, and experiments in stem cells or animal models (mouse and chick), to study the generation of various brain cells. SU18-10
Dr. Leslie Loew R. D. Berlin Center for Cell Analysis and Modeling The project would revolve around a unique software tool, developed here, called SpringSaLaD. It allows realistic modeling and simulation of complex multimolecular interactions in cells using structure-based molecular shapes and data on the rates of biochemical reactions. Depending on the student's interests and skill set, the project would either involve using the software to build a model of a cell signaling pathway, or software engineering to enhance the features of SpringSaLaD. SU18-11
Dr. Nilanjana Maulik Surgery To study the role of exosomes in microsignaling mechanism through microRNA and messenger RNAs between various organs and cell types in Cardiovascular diseases and in Critical limb ischemia. Exosomes are small vesicles that are released by almost every cell type and play a crucial role both in the pathogenesis and therapeutic events depending on the status of the cells. They are important mediators of intercellular communication. The project involves open heart surgery and hind limb ischemia in rodents and high tech isolation procedure of exosomes from plasma. This project also provides the opportunity to learn how to examine the specimen under electron microscopy, confocal microscopy, in vitro and in vivo imaging, imaging of exosomes, isolation of total RNA, miRNA from exosomes, analysis of miRNA and mRNA pool from exosomes, and use of genetic animals to study heart failure and critical limb ischemia--Phase I study. SU18-12
Dr. Ion Moraru Center for Cell Analysis and Modeling This project combines experimental and computational approaches to determine the changes that occur when cells lose DNA mismatch repair function and how it relates to carcinogenesis. Single-cell RNASeq profiling data of stem cell-derived organoids have been used to ab initio the cell subpopulations that develop in the organoids. Dynamic models and simulations of intracellular signaling pathways will be created to characterize the behavior of wild-type and mutant samples. Additionally, new single-cell RNASeq data is expected to be available from adult-derived organoids for identifying subpopulations and comparison to stem cell-derived organoids. SU18-13
Dr. Syam Nukavarapu Orthopaedic Surgery The project we have deals with the characterization of amorphous silica fiber scaffolds and their ability to deliver growth factors. Under this project, the student will learn biomineralization, growth factor loading and controlled release concepts and put them to work to determine the scaffolds' ability to support controlled factor release. The student will carryout the experiments first with a model protein followed by bone morphogenetic protein-2. These studies will help establish scaffold release kinetics and determine the conditions for a sustained and long-term factor release. SU18-14
Dr. Douglas Oliver Neuroscience We will develop an electrophysiological test to demonstrate the presence or absence of tinnitus in animals and human subjects. Over the summer, this will involve electrophysiological recordings from neurons deep in the brains of mice who are exposed to sound. We will study the changes in sound-evoked activity before, during, and after drug-induced tinnitus. SU18-15
Dr. Rishikesh Pandey Pediatrics To study red blood cells' biophysical and biochemical properties using our home-built multi-modal and multi-wavelength system (MMS) featuring Raman micro-spectroscopy, quantitative phase imaging (QPI) and auto-fluorescence imaging. We intend to develop our next generation MMS system equipped with incubator set-up to enable live-cell imaging. The other project centers on label-free and real-time classification of normal lymphocytic B-cells from B-ALL infected cell using our MMS system by studying pathophysiology of live cells by exploiting morphological and molecular information. The ultimate goal is to translate this technology to point of care setting for theranostic applications. SU18-16
Dr. Blanka Rogina Genetics and Genome Sciences Indy (I'm not dead yet) encodes the fly homologue of a mammalian SLC13A5 plasma membrane citrate transporter. Reduction in the Indy gene activity in flies and worms extends their longevity. Decreased INDY expression has beneficial effects on energy balance in worms, flies, mice, and rats. The emerging role of the midgut in healthy aging led us to investigate how INDY reduction in fly intestine influences intestinal stem cell (ISC) homeostasis. The goal of our research is to determine the mechanism underlying beneficial effects of reduced INDY levels on ISC homeostasis and gut integrity. SU18-17
Dr. Stephen Schensul Community Medicine and Health Care Over the last two decades there has been an expanding global epidemic of chronic kidney disease of unknown etiology (CKDu) that has emerged in rural, arid, agricultural lowland regions in multiple countries, including Asia, the Middle East and Central America. Our National Institutes of Health-funded project focuses on identifying factors associated with delaying progression of the disease in Sri Lanka, one of the countries hardest hit by the CKDu epidemic. The project is a collaboration between the University of Connecticut in the US and the University of Peradeniya in Sri Lanka and involves biogeochemists, social scientists and nephrologists from both institutions working collaboratively to identify the environmental, behavioral and health care factors that are associated with the rate of progression from moderate to more advanced CKDu. SU18-18
Dr. Tannin Schmidt Biomedical Engineering Lubricin is a lubricating molecule recently discovered on the eye and in tears that is important for ocular surface health. Recombinant human lubricin has been shown to be clinically effective in improving signs and symptoms of dry eye disease, and is also able to adsorb to commercial contact lenses and reduce friction. This project will examine the biochemical and biomechanical regulation of lubricin biosynthesis by ocular surface cells, and subsequent interaction with contact lenses and contact lens biomaterials. Recently discovered anti-inflammatory properties of lubricin will also be examined within the context of ocular surface cells and tissues. SU18-19
Dr. Henry Smilowitz Cell Biology Our lab is studying heavy atom nanoparticles to enhance radiation therapy of tumors with an emphasis on brain tumor therapy. We also study the synergy of the combination of heavy atom nanoparticle radiation enhancement with other therapies such as chemotherapy and immunotherapy. We also study radiation therapy induced tumor dormancy. The student will take part in a project related to these lab interests. Topics of interest will include the relationship between the distribution of the nanoparticles and the efficacy of radiation enhancement, radiation enhancement and the efficacy of chemo- and immunotherapies, the effect of radiation dose and tumor dormancy. SU18-20
Dr. Ephraim Trakhtenberg Neuroscience We study how the brain develops and utilize gained knowledge to reverse-engineer regeneration of the brain tissue damaged by an injury or stroke. We employ a multidisciplinary approach spanning cutting edge genetics, epigenetics, bioinformatics, molecular biology, and gene therapy, which will provide a student with an opportunity to explore different approaches and select a project that aligns best with the individual’s career goals and interests. SU18-21
Dr. Paola Vera-Licona Center for Quantitative Medicine The student involved in this project will apply computational systems biology and bioinformatics approaches to analyze gene expression RNA-seq data from breast cancer to propose and prioritize combinations of therapeutic targets, that will then be provided to our collaborators for experimental validation. Bioinformatics tools will include the use of the software package geneXplain (http://genexplain.com/) and some R packages to visualize and analyze RNA-seq data. In addition, the student will learn to use some Cytoscape apps (http://www.cytoscape.org/) and customize them to our needs. SU18-22
Dr. Kurutihalli Vishwanatha Neuroscience My lab is interested in molecular mechanisms involved in secretory and endocytic pathways of endocrine cells. One of the project investigates novel roles of PAM, an amidating enzyme of the secretory pathway in exosome secretion. This project is aimed at characterizing the secretion of exosomes by atrial cardiomyocytes derived from WT and conditional PAM knock out mice. SU18-23
Dr. Zhao-Wen Wang Neuroscience An automated worm tracking system (Track-A-Worm) developed in the Wang lab allows quantitative analyses of locomotion behavior of C. elegans, which is very useful to interrogating gene functions. The software component of Track-A-Worm was developed to run with the Matlab in Windows environment. However, it cannot quantify worm locomotion parameters accurately in new versions of Matlab. We need to make the software fully compatible with new Matlab and hope to eventually make Track-A-Worm a standalone system. SU18-24
Dr. Liping Xiao Medicine and Psychiatry This summer project is designed to conduct additional experiments for publication of a manuscript entitled "Microbiota and sickle cell bone disease in mice." Under the supervision of the PI, the student will design primers for quantitative real-time PCR (qPCR), perform qPCR analysis for gene expression, genotyping, histology sectioning and staining, and draft a manuscript. The student could be listed as first author or co-author of the manuscript contingent on satisfactory progress. SU18-25
Dr. Ping Yan Center for Cell Analysis and Modeling The student will have broad hands-on experience on the synthesis of organic compounds, and test the newly developed voltage sensitive dyes using spectroscopic and microscopic methods, on model cell membrane, and even on real cells (in collaboration with Dr. Loew). SU18-26
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Summer 2017 Research Opportunities

Summer 2017 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Andrew Arnold & Dr. Jessica Costa Center for Molecular Medicine We are currently pursuing a number of approaches, including the use of transgenic mouse models and cell lines, to learn more about the precise mechanisms of human tumorigenesis. In addition, we are continuing a major initiative seeking additional genes that contribute to human neoplasia. SU17-1
Dr. Michael Blinov Center for Cell Analysis and Modeling Our Center is developing software for modeling and simulation of biological models. We maintain Virtual Cell (http://vcell.org) - a general web-based framework (written in Java), and interested in providing interfacing with other tools. The student is expected to modify code of SloppyCell (Python/C, http://sloppycell.sourceforge.net/) to provide seamless integration of I/O structure with VCell. Students proficient in Perl can do the same for BioNetFit (http://bionetfit.nau.edu), while C++ developers can work with NFSim (http://nfsim.org). SU17-2
Dr. Caroline Dealy Reconstructive Sciences/Orthopedic Surgery/Regenerative Medicine/Cell Biology The long term goal of the project is to understand and identify growth factor signals that control stem cell responses that mediate limb regeneration. The overarching health care need is to one day develop biological replacements for human limbs lost due to accident or military conflict. Dr Dealy is a developmental biologist/molecular biologist (and UConn alumnus) and her laboratory is using growth factors and stem cells to approach the problem of limb regeneration incompetence in mammals, using a mouse model of digit loss. SU17-3
Dr. Mallika Ghosh Center for Vascular Biology CD13 is a multifunctional cell surface peptidase that is expressed on a variety of cells where we have shown that it modulates receptor-mediated endocytosis and ligand internalization to control downstream signaling pathways. We will explore how CD13 regulates receptor endocytosis and recycling of two major receptors S1P1 receptor and beta1 Integrin receptor which are involved in fundamental cellular function. We will track the receptor by immunostaining followed by fluorescence microscopy. The two main projects are as follows: 1. CD13 mediated regulation of endocytosis and recycling of G-protein coupled receptor, S1P1 receptor induced by its ligand S1P/FTY-P to regulate cytoskeletal remodeling in vitro and in vivo. 2. Regulation of beta1 Integrin recycling, cell migration and focal adhesion turnover by CD13 in cancer epithelial cells. SU17-4
Dr. Arthur Gunzl Genetics and Genome Sciences We work on trypanosomes which cause neglected tropical diseases (our strain is not pathogenic to humans). We are interested in how gene expression in these single cell eukaryotes is different from human cells. We have identified a number of proteins that seem to be parasite-specific gene expression factors. Project options will be to silence a gene of such a factor and analyze its effect on trypanosome viability and gene expression, or to express the factor with a tag in the parasite and determine whether it functions in a protein complex. SU17-5
Dr. Sivapriya Kailasan Vanaja Immunology The project will focus on identifying enterohemorrhagic Escherichia coli (EHEC) virulence factors that modify innate immune responses. It will involve screening mutants of EHEC deficient in virulence factors for their ability to induce toll like receptor (TLR)- and inflammasome-mediated responses. Once a mutant is identified, the project will focus on determining the mechanism of immune modulation. SU17-6 SUMMER ONLY
Dr. Insoo Kim Medicine In this project the student will participate in development of a wearable sensor for blood pressure monitoring. The nature of this project will require the student to learn several engineering expertise such as biomedical instrumentation, 3D printing work for human phantom development, digital signal processing. The student will be asked to do a human phantom development using a 3D printer, electronic circuit board building, and lab experiments with the sensor. SU17-7
Dr. Min Jung Kim Calhoun Cardiology Center Cardiovascular Disease IOS App Development We want to develop an IOS app based on the HealthKit, ResearchKit, and CareKit frameworks that Apple provides. The app is focused on the patients with cardiovascular disease. The use of our app enables patients to better understand the value of health monitoring and to facilitate communicating with the doctor. SU17-8 SUMMER ONLY
Dr. Reinhard Laubenbacher Center for Quantitative Medicine The Center for Quantitative Medicine at UConn Health is developing a software platform for computational algorithms related to medicine and healthcare. The platform is based on the relatively new technology of Docker software containers, which can be used to share environment-independent computational, mathematical, and statistical tools. The site algorun.org contains more information. Qualified undergraduate students will participate in the further development of this platform, learn novel software engineering techniques, become proficient in Docker applications, and participate in providing software solutions to problems in the health field. SU17-9
Dr. Leslie Loew R. D. Berlin Center for Cell Analysis and Modeling We are developing modules, called Model Bricks, that will quantitatively specify fundamental molecular mechanisms controlling cell physiology. These will be combined to develop complex models of cell behavior. SU17-10
Dr. Xin-Ming Ma Neuroscience Schizophrenia is a serious mental illness. The mechanisms underlying schizophrenia are poorly understood. Current understanding of schizophrenia has been generated from neuroimaging analysis, genetic studies, postmortem tissue analysis, and animal models, which all have their limitations. Patient-derived induced pluripotent stem cells (iPSCs) provide an excellent platform for exploring disease mechanisms in a way animal models cannot. Our lab is using iPSC-derived neurons from schizophrenic patients and healthy controls to study the mechanisms underlying schizophrenia. The specific aim is to compare the dendritic spines and synapses in iPSC-derived neurons between schizophrenic patients and healthy controls. SU17-11
Dr. David Martinelli Neuroscience The undergraduate research project will involve the study of the C1q-like family of proteins, which are secreted from neurons of the brain and contribute to the formation and maintenance of synapses. A variety of techniques will be taught, ranging from biochemistry to in vitro cell culture to rodent behavior assays. There are multiple possibilities for which neuronal circuit to focus on, including the auditory system with disease relevance for sound-induced hearing loss, and prefrontal cortex circuitry, with disease relevance for addiction and ADHD. In addition to gaining experience in laboratory techniques, the student will learn the critical thinking skills required of a modern laboratory scientist. SU17-12
Dr. Nilanjana Maulik Surgery Cardiovascular diseases (CVDs) remain among the deadliest diseases in the world, standing next to cancer and stroke. Among the major CVDs, acute myocardial infarction (AMI) is a key life threatening disorder that occurs due to permanent damage of the left ventricular cardiac tissue. The major coronary arteries that supply blood to the functional left ventricle get blocked due to thrombotic plaque occlusion. Therefore, the long-term objective of my laboratory is to design strategies to prevent heart failure with modified stem cells, growth factors, scaffolds, and exosomal nano vesicles to induce therapeutic angiogenesis and arteriogenesis to increase blood flow, prevent ischemic injury, and preserve ventricular function. SU17-13
Dr. Pedro Mendes Center for Quantitative Medicine Selection of software architecture for creating multi-scale models of iron biochemistry. In our research group we have available models of iron biochemistry at different levels of representation: a) whole body and b) specific cell types. We now wish to combine these into a multi-scale model that includes both scales. There are already existing software applications that can do this in principle and we are interested in testing how these applications work. SU17-14 SUMMER ONLY
Dr. Ion Moraru Cell Biology and Center for Cell Analysis and Modeling This project combines experimental and computational approaches to determine the changes that occur when cell lose DNA mismatch repair function and how it relates to carcinogenesis. Single-cell RNASeq profiling data of stem cell-derived organoids will be analyzed to identify ab initio all the cell subpopulations that develop. Dynamic models and simulations of intracellular signaling pathways will be created to characterize the behavior of wild-type and mutant samples. SU17-15
Dr. Julie Robison Center on Aging The student will be trained to conduct telephone and in-person interviews of participants in the CT Money Follows the Person Rebalancing Demonstration (MFP) who have transitioned from nursing homes or other institutions to community settings. The student will also be trained to conduct interviews with unpaid family caregivers of the MFP participants. Tasks include tracking of difficult to find research participants, data collection and entry, data cleaning and analysis, and general collaboration with research team members, community organizations, and research participants to facilitate all aspects of the research effort. SU17-16
Dr. Blanka Rogina Genetics and Genome Sciences Indy (I'm not dead yet) encodes the fly homologue of a mammalian SLC13A5 plasma membrane citrate transporter. Decreased INDY expression in flies and its homologue in worms extend longevity. Reduced Indy gene activity has beneficial effects on energy balance in mice, worms, and flies. The goal of the research is to study the mechanism underlying beneficial effects of reduced INDY levels on intestinal stem cell (ISC) homeostasis. SU17-17
Professor James Schaff Center for Cell Analysis and Modeling Our Center is developing and maintaining a distributed multi-user modeling and simulation environment for cell biology (http://vcell.org). We are interested in developing a new capability focused on model assisted analysis of microscopy experiments. This project involves developing spatial models in VCell to mathematically model specific microscopy experiments to improve the quantitative analysis of experimental data and gain new insight into the underlying biology. The specific biological system involves translation experiments of optogenetic ligands with photoactivatable membrane receptors. This project will focus on developing methodology to couple spatial modeling and image analysis and will involve some light software development. SU17-18
Dr. Stephen Schensul Community Medicine and Health Care This NIH-funded project (2016-2018) focuses on Chronic Kidney Disease of Unknown Etiology (CKDu), not generated by diabetes or hypertension, that is characteristic of standard CKD. CKDu is prevalent in many parts of the world and is endemic among farmers (30-60 years of age) in rural areas of Sri Lanka. CKDu is progressive, with limited treatment options, and leads to End-Stage Renal Disease (ESRD) requiring dialysis or a kidney transplant, both a limited resource in low and middle income countries. The project is concerned with identifying the environmental, behavioral, medical and genetic factors that may contribute to slowing the progression of CKDu. SU17-19
Dr. Henry Smilowitz Cell Biology Our lab collaborates with a small biotech company to study heavy atom nanoparticles as both blood pool contrast agents and as enhancers of radiation therapy for tumor therapy. Our lab also studies the combination of radiation therapy and immunotherapy for tumor therapy with and emphasis on brain tumor therapy. We are currently studying a novel heavy atom nanoparticle for its translation potential to patients for applications related to the above utilities. Any student interested in these types of studies should get in touch with Dr. Smilowitz to discuss specific, timely projects. SU17-20
Dr. Ephraim Trakhtenberg Neuroscience We study how the brain develops and utilize gained knowledge to reverse-engineer regeneration of the brain tissue damaged by an injury or stroke. We employ a multidisciplinary approach spanning cutting edge genetics, epigenetics, bioinformatics, molecular biology, and gene therapy, which will provide a student with an opportunity to explore different approaches and select a project that aligns best with the individual’s career goals and interests. SU17-21
Dr. Kurutihalli Vishwanatha Neuroscience The project is aimed at understanding the role of the proton pump (V-ATPase) in the development and maintenance of secretory pathways in neurons and neuroendocrine cells. The proton pump is responsible for the luminal acidification of the vesicles and also other organelles of the secretory and endocytic pathways like Golgi, endosomes and lysosomes. The activity of the proton pump is spatially and temporally regulated to support the biochemical actions of the enzymes of these pathways. The project will include investigating the macromolecular interactions of V-ATPase with other regulatory accessory proteins of the secretory and endocytic pathways of neuroendocrine cells. SU17-22
Dr. George Wu Medicine We have recently discovered a method by which functional mitochondria can be internalized by a specific cell type through receptor-mediated endocytosis, and replace damaged or absent mitochondria. Our previous studies were dependent on co-localization of complexed mitochondria and a targeted endosomolytic agent into the same endosomes in order to achieve endosomal escape of targeted mitochondria. We wondered whether covalent linkage of an endosomolytic agent directly to the targeting conjugate could increase the efficiency of mitochondrial internalization and escape. The proposed project would include chemical linkage of a targetable carrier protein to a bacterial peptide, and preparation of an affinity chromatography system to purify the ternary conjugates. Using mitochondria from mouse cells as donors, and human hepatoma cells as recipients, uptake assays will be performed to test the new conjugates by confocal microscopy using fluorescent anti-mouse mitochondrial aby, and qPCR using primers specific for mouse mitochondrial DNA relative to human, LDHA chromosomal gene levels. SU17-23
Dr. Liping Xiao Medicine and Psychiatry This summer project is designed to conduct additional experiments for publication of a manuscript entitled "FOXO3 regulates bone formation of BMSC in vitro from sickle cell disease mice." Under the supervision of the PI, the student will design primers for quantitative real-time PCR (qPCR), perform qPCR analysis for gene expression, and draft the manuscript. The student could be listed as first author or co-author of the manuscript contingent on satisfactory progress. SU17-24

Academic Year 2017-18 Research Opportunities

Academic Year 2017-18 Research Opportunities

Academic Year 2017-18 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Andrew Arnold & Dr. Jessica Costa Center for Molecular Medicine We are currently pursuing a number of approaches, including the use of transgenic mouse models and cell lines, to learn more about the precise mechanisms of human tumorigenesis. In addition, we are continuing a major initiative seeking additional genes that contribute to human neoplasia. AY1718-1
Dr. Mayu Inaba Cell Biology Drosophila genetic crosses. Perform basic immuno-histochemical experiments (preparation of melanogaster tissues for imaging and confocal microscope imaging). Live imaging of stem cell division, behavior of cellular organelle and cell-cell communications. Molecular cloning and mutagenesis in E coli and preparation of vectors for transgenesis in D. melanogaster. AY1718-5
Dr. Carol Pilbeam Medicine and Orthopaedics; Director of MD/PhD Program Our lab has found a new pro-inflammatory factor, serum amyloid A (SAA), that can regulate bone turnover. SAA is only produced when prostaglandins (PGs) are also expressed. We generated an SAA knockout mouse and found that SAA is responsible for a significant amount of bone loss with aging in mice. Our projects are focused on determining the mechanisms by which SAA causes bone loss, how SAA is regulated by PGS, and the general role of SAA in inflammatory responses by bone marrow macrophages. AY1718-2
Dr. Ephraim Trakhtenberg Neuroscience We study how the brain develops and utilize gained knowledge to reverse-engineer regeneration of the brain tissue damaged by an injury or stroke. We employ a multidisciplinary approach spanning cutting edge genetics, epigenetics, bioinformatics, molecular biology, and gene therapy, which will provide a student with an opportunity to explore different approaches and select a project that aligns best with the individual’s career goals and interests. AY1718-3
Dr. Paola Vera-Licona Center for Quantitative Medicine The student involved in this project will apply computational systems biology and bioinformatics approaches to analyze gene expression RNA-seq data from Neuroblastoma cell lines to propose and prioritize combinations of therapeutic targets, that will then be provided to our collaborators for experimental validation. Bioinformatics tools will include the use of the software package geneXplain (http://genexplain.com/) and some R packages to visualize and analyze RNA-seq data. In addition, the student will learn to use some Cytoscape apps (http://www.cytoscape.org/) and customize them to our needs. AY1718-6 2/28 DEADLINE
Dr. Beiyan Zhou Immunology We are accepting highly motivated undergraduate students to join the lab to enrich their research experience in the area of immunology/hematopoiesis/ obesity/stem cells. Our research projects incorporate epigenetic and genomic approaches to understand the crosswalks between immune compartment and host tissue cells under chronic stress conditions, such as obesity. AY1718-4

Summer 2017 Research Opportunities

Summer 2017 Research Opportunities

Summer 2017 applications are closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Andrew Arnold & Dr. Jessica Costa Center for Molecular Medicine We are currently pursuing a number of approaches, including the use of transgenic mouse models and cell lines, to learn more about the precise mechanisms of human tumorigenesis. In addition, we are continuing a major initiative seeking additional genes that contribute to human neoplasia. SU17-1
Dr. Michael Blinov Center for Cell Analysis and Modeling Our Center is developing software for modeling and simulation of biological models. We maintain Virtual Cell (http://vcell.org) - a general web-based framework (written in Java), and interested in providing interfacing with other tools. The student is expected to modify code of SloppyCell (Python/C, http://sloppycell.sourceforge.net/) to provide seamless integration of I/O structure with VCell. Students proficient in Perl can do the same for BioNetFit (http://bionetfit.nau.edu), while C++ developers can work with NFSim (http://nfsim.org). SU17-2
Dr. Caroline Dealy Reconstructive Sciences/Orthopedic Surgery/Regenerative Medicine/Cell Biology The long term goal of the project is to understand and identify growth factor signals that control stem cell responses that mediate limb regeneration. The overarching health care need is to one day develop biological replacements for human limbs lost due to accident or military conflict. Dr Dealy is a developmental biologist/molecular biologist (and UConn alumnus) and her laboratory is using growth factors and stem cells to approach the problem of limb regeneration incompetence in mammals, using a mouse model of digit loss. SU17-3
Dr. Mallika Ghosh Center for Vascular Biology CD13 is a multifunctional cell surface peptidase that is expressed on a variety of cells where we have shown that it modulates receptor-mediated endocytosis and ligand internalization to control downstream signaling pathways. We will explore how CD13 regulates receptor endocytosis and recycling of two major receptors S1P1 receptor and beta1 Integrin receptor which are involved in fundamental cellular function. We will track the receptor by immunostaining followed by fluorescence microscopy. The two main projects are as follows: 1. CD13 mediated regulation of endocytosis and recycling of G-protein coupled receptor, S1P1 receptor induced by its ligand S1P/FTY-P to regulate cytoskeletal remodeling in vitro and in vivo. 2. Regulation of beta1 Integrin recycling, cell migration and focal adhesion turnover by CD13 in cancer epithelial cells. SU17-4
Dr. Arthur Gunzl Genetics and Genome Sciences We work on trypanosomes which cause neglected tropical diseases (our strain is not pathogenic to humans). We are interested in how gene expression in these single cell eukaryotes is different from human cells. We have identified a number of proteins that seem to be parasite-specific gene expression factors. Project options will be to silence a gene of such a factor and analyze its effect on trypanosome viability and gene expression, or to express the factor with a tag in the parasite and determine whether it functions in a protein complex. SU17-5
Dr. Sivapriya Kailasan Vanaja Immunology The project will focus on identifying enterohemorrhagic Escherichia coli (EHEC) virulence factors that modify innate immune responses. It will involve screening mutants of EHEC deficient in virulence factors for their ability to induce toll like receptor (TLR)- and inflammasome-mediated responses. Once a mutant is identified, the project will focus on determining the mechanism of immune modulation. SU17-6 SUMMER ONLY
Dr. Insoo Kim Medicine In this project the student will participate in development of a wearable sensor for blood pressure monitoring. The nature of this project will require the student to learn several engineering expertise such as biomedical instrumentation, 3D printing work for human phantom development, digital signal processing. The student will be asked to do a human phantom development using a 3D printer, electronic circuit board building, and lab experiments with the sensor. SU17-7
Dr. Min Jung Kim Calhoun Cardiology Center Cardiovascular Disease IOS App Development We want to develop an IOS app based on the HealthKit, ResearchKit, and CareKit frameworks that Apple provides. The app is focused on the patients with cardiovascular disease. The use of our app enables patients to better understand the value of health monitoring and to facilitate communicating with the doctor. SU17-8 SUMMER ONLY
Dr. Reinhard Laubenbacher Center for Quantitative Medicine The Center for Quantitative Medicine at UConn Health is developing a software platform for computational algorithms related to medicine and healthcare. The platform is based on the relatively new technology of Docker software containers, which can be used to share environment-independent computational, mathematical, and statistical tools. The site algorun.org contains more information. Qualified undergraduate students will participate in the further development of this platform, learn novel software engineering techniques, become proficient in Docker applications, and participate in providing software solutions to problems in the health field. SU17-9
Dr. Leslie Loew R. D. Berlin Center for Cell Analysis and Modeling We are developing modules, called Model Bricks, that will quantitatively specify fundamental molecular mechanisms controlling cell physiology. These will be combined to develop complex models of cell behavior. SU17-10
Dr. Xin-Ming Ma Neuroscience Schizophrenia is a serious mental illness. The mechanisms underlying schizophrenia are poorly understood. Current understanding of schizophrenia has been generated from neuroimaging analysis, genetic studies, postmortem tissue analysis, and animal models, which all have their limitations. Patient-derived induced pluripotent stem cells (iPSCs) provide an excellent platform for exploring disease mechanisms in a way animal models cannot. Our lab is using iPSC-derived neurons from schizophrenic patients and healthy controls to study the mechanisms underlying schizophrenia. The specific aim is to compare the dendritic spines and synapses in iPSC-derived neurons between schizophrenic patients and healthy controls. SU17-11
Dr. David Martinelli Neuroscience The undergraduate research project will involve the study of the C1q-like family of proteins, which are secreted from neurons of the brain and contribute to the formation and maintenance of synapses. A variety of techniques will be taught, ranging from biochemistry to in vitro cell culture to rodent behavior assays. There are multiple possibilities for which neuronal circuit to focus on, including the auditory system with disease relevance for sound-induced hearing loss, and prefrontal cortex circuitry, with disease relevance for addiction and ADHD. In addition to gaining experience in laboratory techniques, the student will learn the critical thinking skills required of a modern laboratory scientist. SU17-12
Dr. Nilanjana Maulik Surgery Cardiovascular diseases (CVDs) remain among the deadliest diseases in the world, standing next to cancer and stroke. Among the major CVDs, acute myocardial infarction (AMI) is a key life threatening disorder that occurs due to permanent damage of the left ventricular cardiac tissue. The major coronary arteries that supply blood to the functional left ventricle get blocked due to thrombotic plaque occlusion. Therefore, the long-term objective of my laboratory is to design strategies to prevent heart failure with modified stem cells, growth factors, scaffolds, and exosomal nano vesicles to induce therapeutic angiogenesis and arteriogenesis to increase blood flow, prevent ischemic injury, and preserve ventricular function. SU17-13
Dr. Pedro Mendes Center for Quantitative Medicine Selection of software architecture for creating multi-scale models of iron biochemistry. In our research group we have available models of iron biochemistry at different levels of representation: a) whole body and b) specific cell types. We now wish to combine these into a multi-scale model that includes both scales. There are already existing software applications that can do this in principle and we are interested in testing how these applications work. SU17-14 SUMMER ONLY
Dr. Ion Moraru Cell Biology and Center for Cell Analysis and Modeling This project combines experimental and computational approaches to determine the changes that occur when cell lose DNA mismatch repair function and how it relates to carcinogenesis. Single-cell RNASeq profiling data of stem cell-derived organoids will be analyzed to identify ab initio all the cell subpopulations that develop. Dynamic models and simulations of intracellular signaling pathways will be created to characterize the behavior of wild-type and mutant samples. SU17-15
Dr. Julie Robison Center on Aging The student will be trained to conduct telephone and in-person interviews of participants in the CT Money Follows the Person Rebalancing Demonstration (MFP) who have transitioned from nursing homes or other institutions to community settings. The student will also be trained to conduct interviews with unpaid family caregivers of the MFP participants. Tasks include tracking of difficult to find research participants, data collection and entry, data cleaning and analysis, and general collaboration with research team members, community organizations, and research participants to facilitate all aspects of the research effort. SU17-16
Dr. Blanka Rogina Genetics and Genome Sciences Indy (I'm not dead yet) encodes the fly homologue of a mammalian SLC13A5 plasma membrane citrate transporter. Decreased INDY expression in flies and its homologue in worms extend longevity. Reduced Indy gene activity has beneficial effects on energy balance in mice, worms, and flies. The goal of the research is to study the mechanism underlying beneficial effects of reduced INDY levels on intestinal stem cell (ISC) homeostasis. SU17-17
Dr. James Schaff Center for Cell Analysis and Modeling Our Center is developing and maintaining a distributed multi-user modeling and simulation environment for cell biology (http://vcell.org). We are interested in exposing VCell's simulation and data analysis capabilities through a Python API communicating with our local VCell client (java) and our remote RESTful VCell API. We will use this capability to provide a IPython/Jupyter notebook for the interactive scripting of VCell for running simulations and performing data analysis with VCell. SU17-18
Dr. Stephen Schensul Community Medicine and Health Care This NIH-funded project (2016-2018) focuses on Chronic Kidney Disease of Unknown Etiology (CKDu), not generated by diabetes or hypertension, that is characteristic of standard CKD. CKDu is prevalent in many parts of the world and is endemic among farmers (30-60 years of age) in rural areas of Sri Lanka. CKDu is progressive, with limited treatment options, and leads to End-Stage Renal Disease (ESRD) requiring dialysis or a kidney transplant, both a limited resource in low and middle income countries. The project is concerned with identifying the environmental, behavioral, medical and genetic factors that may contribute to slowing the progression of CKDu. SU17-19
Dr. Henry Smilowitz Cell Biology Our lab collaborates with a small biotech company to study heavy atom nanoparticles as both blood pool contrast agents and as enhancers of radiation therapy for tumor therapy. Our lab also studies the combination of radiation therapy and immunotherapy for tumor therapy with and emphasis on brain tumor therapy. We are currently studying a novel heavy atom nanoparticle for its translation potential to patients for applications related to the above utilities. Any student interested in these types of studies should get in touch with Dr. Smilowitz to discuss specific, timely projects. SU17-20
Dr. Ephraim Trakhtenberg Neuroscience We study how the brain develops and utilize gained knowledge to reverse-engineer regeneration of the brain tissue damaged by an injury or stroke. We employ a multidisciplinary approach spanning cutting edge genetics, epigenetics, bioinformatics, molecular biology, and gene therapy, which will provide a student with an opportunity to explore different approaches and select a project that aligns best with the individual’s career goals and interests. SU17-21
Dr. Kurutihalli Vishwanatha Neuroscience The project is aimed at understanding the role of the proton pump (V-ATPase) in the development and maintenance of secretory pathways in neurons and neuroendocrine cells. The proton pump is responsible for the luminal acidification of the vesicles and also other organelles of the secretory and endocytic pathways like Golgi, endosomes and lysosomes. The activity of the proton pump is spatially and temporally regulated to support the biochemical actions of the enzymes of these pathways. The project will include investigating the macromolecular interactions of V-ATPase with other regulatory accessory proteins of the secretory and endocytic pathways of neuroendocrine cells. SU17-22
Dr. George Wu Medicine We have recently discovered a method by which functional mitochondria can be internalized by a specific cell type through receptor-mediated endocytosis, and replace damaged or absent mitochondria. Our previous studies were dependent on co-localization of complexed mitochondria and a targeted endosomolytic agent into the same endosomes in order to achieve endosomal escape of targeted mitochondria. We wondered whether covalent linkage of an endosomolytic agent directly to the targeting conjugate could increase the efficiency of mitochondrial internalization and escape. The proposed project would include chemical linkage of a targetable carrier protein to a bacterial peptide, and preparation of an affinity chromatography system to purify the ternary conjugates. Using mitochondria from mouse cells as donors, and human hepatoma cells as recipients, uptake assays will be performed to test the new conjugates by confocal microscopy using fluorescent anti-mouse mitochondrial aby, and qPCR using primers specific for mouse mitochondrial DNA relative to human, LDHA chromosomal gene levels. SU17-23
Dr. Liping Xiao Medicine and Psychiatry This summer project is designed to conduct additional experiments for publication of a manuscript entitled "FOXO3 regulates bone formation of BMSC in vitro from sickle cell disease mice." Under the supervision of the PI, the student will design primers for quantitative real-time PCR (qPCR), perform qPCR analysis for gene expression, and draft the manuscript. The student could be listed as first author or co-author of the manuscript contingent on satisfactory progress. SU17-24

Spring 2017 Research Opportunities

Spring 2017 Research Opportunities

Spring 2017 applications are now closed. For reference purposes, the names of participating faculty and a brief description of the research opportunities they sponsored for Spring 2017 appear in the table below. Click on the relevant link in the right column to view more detail about the opportunity.
Faculty Mentor Project Description Opportunity Link
Dr. Andrew Arnold & Dr. Jessica Costa Center for Molecular Medicine We are currently pursuing a number of approaches, including the use of transgenic mouse models and cell lines, to learn more about the precise mechanisms of human tumorigenesis. In addition, we are continuing a major initiative seeking additional genes that contribute to human neoplasia. HRP15
Dr. Ernesto Canalis Orthopaedics and Medicine Notch receptors are determinant of cell fate and function. Our laboratory has studied the function of Notch for the past 15 years. We have developed mouse models of human diseases associated with Notch mutation, specifically mice engineered to carry mutations associated with Hajdu Cheney and Lateral Meningocele Syndromes. We suspect that these mouse mutants have developmental defects of the skeleton. The project entails the characterization of the skeleton during embryonic development using histological and molecular approaches. HRP1
Dr. Audrey Chapman Community Medicine Stem cell research is considered one of the most promising fields of medicine. I am engaged in a research project identifying the challenges of translating pluripotent stem cell research into therapies. The initial work on the project is to track the role of the four state funding programs that have provided much of the basic stem cell research funding in order to evaluate their priorities and the types of grants they have made. I am also evaluating the first-in-human stage one clinical trials with prospective pluripotent stem cell based therapies and whether they have conformed with the guidelines developed by the International Society for Stem Cell Research. HRP6
Dr. Arvind Chhabra Medicine I am working in the area of human cancer immunology & immunotherapy, and human pluripotent stem cells (hPSC). Among the approaches we are working on are the generation and functional characterization of human tumor antigen specific T cells from natural CD8+ cytolytic T cell (CTL) precursors; the creation of customized CD4+ and CD8+ anti-tumor T cells by engineering human peripheral blood derived T cells with a human melanoma-associated antigen specific transgenic T cell receptor (TCR); and the generation of donor-specific induced pluripotent stem cells (iPSC) lines for generating donor specific T cell and antigen presenting cells (APC). I welcome undergraduate researchers to join me in either of the two projects listed below:
  • (1) Characterization of the biology of anti-tumor T cells.
  • (2) Understanding the development of innate and adaptive immune mechanisms in the human immune system utilizing donor-specific iPSC lines as a model.
 HRP13
Dr. Anne Delany Center for Molecular Medicine We use in vitro and in vivo models understand how specific microRNAs (miRNAs, small non-coding RNAs) control the biology of osteoclasts (bone resorbing cells) and osteoblasts (bone forming cells). Projects available for the Spring include understanding how over expression or knockdown of a specific miRNA can alter the differentiation and function of osteoblasts or osteoclasts. Studies will be performed in vitro, using cell lines transduced with lentivirus. In addition, the function of specific miRNAs in vivo will be examined using transgenic mouse models, in which skeletal phenotyping will be performed. Techniques to be used include cell culture, Western blot analysis, qRT-PCR, and histology. HRP10
Dr. Golda Ginsburg Psychiatry Students will be part of a vibrant clinical research lab that conducts studies funded by the National Institute of Mental Health and Department of Education aimed at testing different psychotherapies for youth with anxiety and depression. Students will develop basic research skills including but not limited to collecting and entering data and assisting with assessments with participants. They’ll have the opportunity to become familiar with research protocols and procedures of several randomized controlled trials (RCTs) and the administration of validated psychometric assessments with youth and parents. HRP7
Dr. Damion Grasso Psychiatry The student will be involved in a pilot study funded by the Connecticut Institute of Clinical and Translational Science (CICATS) designed to examine emotional and biological stress reactivity in young children (4-6 years of age) with a history of domestic violence (DV) exposure. Mother-child dyads will be recruited from areawide DV shelters. Data collected with include diagnostic and observational assessments, computer tasks designed to measure attention bias to threat, and physiological measurements (i.e., heart rate reactivity/variability, skin conductance) to threat-related images. A primary aim will be to examine relationships between indicators of stress reactivity and emerging symptoms of posttraumatic stress disorder in these young children. HRP9
Dr. Rosa Guzzo Neuroscience Research in the Guzzo lab is focused on understanding the role of epigenetic modifiers in the development and maintenance of cartilage. To address this question, we employ gain and loss of function studies in human pluripotent stem cells as well as animal models. Research projects in the lab will focus on evaluating the joint phenotype in developing and postnatal mice lacking the expression of a chromatin modifier in cartilage. These studies will employ histological and immunohistochemical staining of joint cartilage tissue from genetically altered mice. Additional mechanistic studies, involving quantitative gene expression analyses and molecular assays, will be used to determine whether small molecules can promote the differentiation of embryonic progenitors and human stem cells to the chondrogenic lineage. HRP16
Dr. Christopher Heinen Center for Molecular Medicine Our laboratory uses human intestinal organoids as a model to study the effects of cancer causing mutations on intestinal stem cells. In the current project, the undergraduate student would work in partnership with members of the lab to create, maintain and characterize these organoids that have been genetically engineered using CRISPR/Cas9 to carry different mutations in the DNA mismatch repair (MMR) genes. This project will involve learning advanced cell culture techniques, molecular biology techniques such as RT-PCR and cell biology techniques such as immunofluorescence microscopy. HRP3
Dr. Liisa Kuhn Reconstructive Sciences/Biomedical Engineering In this project the student will learn how to prepare combinations of biomaterials and biologically active molecules that can be injected into a cartilaginous growth plate to improve healing after injury. This type of work builds on chemistry and biomaterials courses the student may have had. In this project we will also inject materials into mice and then image them by X-ray to understand changes to the bones and cartilage. HRP18
Dr. Sangamesh Kumbar Orthopaedic Surgery Students will be involved in one of two projects: Project 1. Design, fabrication and characterization of polymeric micro-nanostructures for tissue engineering applications. Micro-nanostructures fabrication will utilize 3D printing and advanced electrospinning techniques to design scaffolds for the repair and regeneration of neural and tendon tissues. Scaffolds will be optimized for physicochemical properties in terms of mechanical strength, degradation, and pore and surface properties. Project 2. Polymeric nano-particles for therapeutic and diagnostic applications. We have synthesized and characterized novel fluorescent polymers for this application. Students working on this project will work on per-formulation studies focused on improving the solubility of poorly soluble drugs to improve their bioavailability. Students will learn fabrication, characterization of nano-particles for physico-chemical properties, and drug release behavior. HRP17
Dr. Kevin Manning Psychiatry/Center on Aging Neuropsychology of Older Drivers: Looking for interested undergraduates to assist in a research study on cognitive and automobile driving assessment in older adults. The aim of this work is to: 1) understand the earliest driving changes in older adults who will eventually develop cognitive decline and 2) determine whether driving simulation can be a useful tool in the driving assessment of older drivers. The student will learn how to administer, score, and interpret detailed geriatric cognitive assessments in older adults with mild cognitive impairment. Trainees will develop proficiency in data-analysis using SPSS and Excel and become familiar with driving (using driving simulation and on-road tests) and other functional assessments of everyday activities. Education on the conceptual and day-to-day logistical aspects of running a research study in an academic medical setting will be provided. The student will interact with the primary investigator and a larger team of physicians through the Center on Aging and Department of Psychiatry. HRP2
Dr. Nilanjana Maulik Surgery Cardiovascular diseases (CVDs) remain among the deadliest diseases in the world, standing next to cancer and stroke. Among the major CVDs, acute myocardial infarction (AMI) is a key life threatening disorder that occurs due to permanent damage of the left ventricular cardiac tissue. The major coronary arteries that supply blood to the functional left ventricle get blocked due to thrombotic plaque occlusion. Therefore, the long-term objective of my laboratory is to design strategies to prevent heart failure with modified stem cells, growth factors, scaffolds, and exosomal nano vesicles to induce therapeutic angiogenesis and arteriogenesis to increase blood flow, prevent ischemic injury, and preserve ventricular function. HRP4
Dr. Lakshmi Nair Orthopaedic Surgery Over the years, our studies have led to the development of injectable hydrogels from proteins and polysaccharides. The proposed studies will evaluate various processing parameters to modulate the physical and biological properties of the injectable hydrogels for regenerative applications. These include chemical modification of the existing injectable hydrogels and or modulating the physical and mechanical properties using various processing conditions. HRP14
Dr. Douglas Oliver Neuroscience We will use genetically modified mouse strains to investigate the neurons and circuitry of the inferior colliculus, a major nucleus in the auditory system. We are particularly interested in a mouse where MEK1 is overexpressed resulting in a much larger than normal inferior colliculus. The work includes genotyping mice (PCR), electrophysiological testing of hearing and sound processing (evoked potential or single cell recordings), as well as the morphological assessment of the neuronal network (microscopy and immunohistochemistry). We expect the student to assist in these experiments and animal husbandry. Depending on skill set and research interests, the student will be involved predominately in either histology or electrophysiology experiments. HRP8
Dr. Blanka Rogina Genetics & Genome Sciences The Drosophila I’m Not Dead Yet (INDY) gene encodes a plasma membrane citrate transporter. We have reported that reduction in the Indy gene activity in flies modulates metabolism and extends longevity in a way that has been compared to caloric restriction. Our Indy flies live longer and are protected from the negative effects of high calorie diet. We also reported that INDY reduction in fly intestine preserves intestinal stem cell (ISC) homeostasis. We are currently investigating effect of different calorie content of food on ISC proliferation and how Indy reduction affects this response. HRP12
Dr. Daniel Rosenberg Center for Molecular Medicine Variety of ongoing projects related to GI cancers; epigenetic studies of the DNA methylome, microbiome studies, chemoprevention studies, mouse models of cancer, bioactive lipid signaling, bioinformatics and RNA seq studies. HRP11
Dr. Rajkumar Verma Neuroscience A primary focus of my lab is to understand post-stroke depression (PSD), which is the most common neuropsychiatric consequence of stroke. PSD can impair functional recovery, which can, in turn, reduce the quality of life, especially among the aged population. However, PSD remains understudied compared to other stroke complications such as a motor, cognitive, or language impairments. Elderly patients fare poorly on stroke outcomes compared to their younger counterparts, a phenotype we have recapitulated in our animal models of stroke. Furthermore, female subjects are significantly more likely to develop PSD and to suffer from a more severe form of PSD compared to age-matched males, which is mirrored in rodent models. Compared to young cohorts, aging mice exhibit exaggerated inflammatory and cytokine responses following middle cerebral artery occlusion (MCAO), the most common rodent model of stroke. Increased inflammation activates the peripheral innate immune system, which in turn triggers multiple downstream effects including exacerbated depressive-like behaviors. Age-specific treatment with available antidepressants in clinical PSD populations is highly limited. Moreover, unfavorable outcome such increased hemorrhagic transformation and high mortality in middle-aged adults with the used of fluoxetine, the most common used antidepressant, for PSD treatment further highlights the need to explore relevant, efficacious and safe therapeutic options for PSD in a validated aged animal models to understand their specific mechanism and safety profiles before moving forward with clinical trials. In this project, we will test post-stroke aged mice in several models of depressive phenotypes, PSD development, and motor deficits during prolonged recovery. We test a new FDA-approved drug for the treatment of depression in aged rodents with features of PSD. This study will shed new insights into the treatment of PSD and determine whether this new drug can improve survival, enhance behavioral recovery in aged mice with PSD or not. HRP5