Experimental Design: The graduate student and PI will explain the rationale and objectives of the experiments, providing context for their scientific significance.
Protocol Guidance: A detailed, step-by-step protocol will be provided to the undergraduate student for reference and study.
Demonstration: The graduate student will demonstrate the entire procedure, ensuring the undergraduate student observes and understands each step.
Lab Notebook Review: The PI and graduate student will periodically review the undergraduate student’s lab notebook to ensure accurate and thorough documentation.

Undergraduate Student Responsibilities

Active Feedback: The undergraduate student should communicate openly, asking questions and providing feedback whenever there is confusion or need for clarification.
Engagement in Lab Meetings: Active participation in lab meetings is expected, including presenting progress when appropriate and contributing to discussions.

B. Basic Understanding of Buffers
Students should familiarize themselves with the components and purposes of buffers, including:
1. Buffering Reagents: To maintain pH stability during experiments.
2. Salts: For ionic strength and protein stability.
3. Detergents: To solubilize membrane proteins and stabilize hydrophobic regions.
4. Reductive and Oxidative Reagents: For controlling the redox state of protein samples.

C. Laboratory Practice
Students are expected to maintain a meticulous and detailed lab notebook, documenting all experimental procedures, observations, and results. A well-organized notebook is essential for reproducibility and accurate data interpretation.