Project Mentor
Dr. Xin-Ming Ma
Department of Neuroscience
Undergraduate Research Opportunity Description
| Project Description | Understanding the Role of Interneuron RFWD2 in Depression and Anxiety. Depression frequently co-occurs with anxiety, and the limited understanding of the mechanisms underlying these disorders contributes to the limited effectiveness of currently available antidepressants. RFWD2 has been identified as a risk factor for both conditions, and its protein is expressed in brain regions associated with mood and anxiety regulation. This study aims to determine whether manipulating RFWD2 in interneurons within the mouse brain contributes to the development of depression and/or anxiety in an Rfwd2 genetic mouse model. The findings from this study will enhance our understanding of the mechanisms driving depression and anxiety. |
| Project Direction | Maintaining a proper balance between interneurons and pyramidal neurons is essential for healthy brain function. Disruption of interneuron activity can disturb this balance and may contribute to the development of depression and/or anxiety. In our laboratory, we have generated Rfwd2 conditional knockin (Rfwd2CKIN) mice, in which Rfwd2 is overexpressed in brain interneurons, and Rfwd2 conditional knockout (Rfwd2cko) mice, in which Rfwd2 is selectively deleted in the same interneuron population.
We will use PCR to determine mouse genotypes, and Western blotting to evaluate protein levels in the brain. Behavioral tests will assess depression- and anxiety-like behaviors, as well as spatial learning and memory, in Rfwd2CKIN and Rfwd2cko mice and their wild-type littermate controls. Our long-term goal is to use these mouse models to test our central hypothesis: Interneuron RFWD2 plays a key role in depression and anxiety by modulating the structure and function of dendritic spines in pyramidal neurons. |
| Mentorship and Supervision | The PI and lab members will provide comprehensive training. The student may speak with the PI at any time if questions arise. The PI will monitor research progress on a daily basis and provide timely feedback. The student will be required to read one research article related to their project each week. |
| Student Qualifications | The successful candidate should have a basic understanding of science—such as chemistry, biology, or physiology—as well as foundational knowledge of scientific techniques, including PCR, Western blotting, and reagent or solution preparation. The candidate will also have the opportunity to learn all additional techniques and skills necessary for this project. |
| Summer Schedule Options | Research Dates: May 18 to July 24, 2026 Schedule: Monday-Friday 8am through 5pm or 9am through 5pm |
| Project Continuation | Fall 2026, Spring 2027 |
| Academic Year Time Commitment | 3, 6 hours/week |
| Possible Thesis Project | Yes |
Application
Submit an online application for this research opportunity at https://quest.uconn.edu/prog/HRPSU26-23. The application deadline is Monday, February 16, 2026.
This application requires a cover letter, a resume or CV, an unofficial transcript, GPA and science GPA, a brief statement of research interests, a brief statement of career interests, and references.